Estudo do polimorfismo do sistema sangüíneo Diego em

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Exon 17 Exon 18 Exon 19 Anexo I - (Continuação) Apêndice 9541 ggggcctgta caagcaaagc tccctgaagc tgaagcttcg ttgcctgcga atcagccttg 9601 aatgttgtga agacatttct gctcttaaga gtttggattc tcaagatggg gcgggaggga 9661 ggggagcaag gaactcaaag agcgaggggc acagaagaca aaactgagct acaaggacac 9721 caagtatgga gaaggaagga gggggtggag gaggcagggg agaaccctgc ttacccctca 9781 ccttccccta ccgtaggctg attgtcagca aacctgagcg caagatggtc aagggctccg 9841 gcttccacct ggacctgctg ctggtagtag gcatgggtgg ggtggccgcc ctctttggga 9901 tgccctggct cagtgccacc accgtgcgtt ccgtcaccca tgccaacgcc ctcactgtca 9961 tgggcaaagc cagcacccca ggggctgcag cccagatcca ggaggtcaaa gagcagcgga 10021 tcagtggact cctggtcgct gtgcttgtgg gtgagtgaaa gctgggtcgc gtcttcacca 10081 tcctcctgcc cctcaccccc ggccccttcc ccttcccctt tctgttcgga cccagcacct 10141 cctccagcct cccccatcct gcaccacttc cccacatctc cccagcatcc cctactcaca 10201 cgaggggatc ctggaggaaa ggccaaggca ctgggctggg cgtctgagca DiE17AS gggaggaagg 10261 gggctccact ccgaggcctt ctccagacca gaccccacct tctgacttac ccaacagaag 10321 tccagtgaac tgtgggttct agccctgccc tgctgctaac ccaggatccc tccttgtctc 10381 tgggtctcag tttccctgtc tgcacagttg gagggttcaa cagatgtccc ccagttctct 10441 tccagctcag ctgctccatg actgatacaa ggggcggtgc tctagccata gtccagggat 10501 gtgggggaga agaaagaagg gtcactgggg ttgatttcag ttctggagca gccaagagtc 10561 gagctgggtt cggtgtgggc agtgccatgg ttgcagatgg agaggtcagg gaaaattctt 10621 aaaggaggca gttgatggca ttctgggctg gggtgaggag aaaggtgtgt gtgtttgacc 10681 tctgtgaccc tcattaggtt aagggagtgg gcttaagtca ggcaggcagg gctttgactc 10741 cttgccagct gtgtggcctt aggcaggttg tttacctctc tgagcctgtt tcctcatctg 10801 taaaattggg taatacctat aaaaaggacg cgatagaggc cgggggttgt ggctcacgcc 10861 tgtaatccca gcactttggg agactgaggc aggtgggtca ccctgtccaa catggtgaaa 10921 ccacgtctct actaaaatta caaaatcagc tgggcgtggt gtcgcacgcc tataatccca 10981 gctacttggg aggctgaggc aggagaattg cttgaaccca ggaggcggag gttgaagtga 11041 gctgagattg caccactgca ctccagcctg ggcaacagag tgagactccg cctcaaaaaa 11101 aaaaaaaaaa aaaaaaaaaa agacccatag gaatagctcc aggccaggcg tgggatctca 11161 acactttggg agccaaggca ggagaatcac ttgagcccag gagttcaaga ctagcctggg 11221 caacatacgt gagaccttgt ctctactaaa aattaaaaaa aaaaaaaatg atccaggtgt 11281 ggttgtatgt gcctgtagtt ccagcaacta gggacactga agcgggagga tcacttggga DiEX17S 11341 tcaggaggtt aagtctgttg tgagccgaga tcacactact gcactctggc ttgggtgaca 11401 gagcgagatc ctgtctcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aaaagaaaag 11461 gaaaaaagaa atggctccat atggtgcctg tgttttattc ccagccccag atagggcctg 11521 accccactgg gccaggcctc gggcaacctg ggctgagagt gtgcggctcc DiEX18S cccacaggcc 11581 tgtccatcct catggagccc atcctgtccc gcatccccct ggctgtactg tttggcatct 11641 tcctctacat gggggtcacg tcgctcagcg gcatccagct ctttgaccgc atcttgcttc 11701 tgttcaagcc acccaagtat cacccagatg tgccctacgt caagcgggta caggaccctt 11761 ttctggctgc ccagcctggg gtggtagagg gtgctggggt gtgataggca ctgaccccag 11821 cctccgcctg caggtgaaga cctggcgcat gcacttattc acgggcatcc agatcatctg 11881 cctggcagtg ctgtgggtgg tgaagtccac gccggcctcc ctggccctgc ccttcgtcct 11941 catcctcact gtgccgctgc DiE18ASggcgcgtcct gctgccgctc atcttcagga acgtggagct 12001 tcagtgtgtg agtggctgcc tgggcctggg gcacaagagc tgggagcatg cgaggggcgc 12061 gtctcagaac tagggcaggc aggacagagt catgggtaaa gtctggggct agggctggtg 12121 tccaggttct ggcctgctgt gtcctagcat atgatctgga gcacggggcc ttgtttctct 12181 gtgtcttcat tttgctcacc tgtaaagggg gaaatgatgg cacgactggg DiE19AS gagagcgtca 12241 agaggcagac agggtgtgct gtgagtgagc tggaccccta agcagcaagt cctgccgtct 12301 tccgagtcat ttgggacaga gtcactcctc taataggatt cttccacagg gtgactcagg DiEX19S 12361 tcactgtggg gccgccttgg tgaaaaggtc ccctgctcac cctctgcagg ccctcccctg 12421 ccagggtcca catccccggg gttgcctctc actttgacaa cgactcattt aggccagtcc 12481 tagctgctgg ggagataaaa caggactggt ccaaggttca agacagccag accctctcct 12541 aactccagag gtgcctttcg tgcccgggga gggctcttct ggccccaggg tctctcaccc 12601 tgtctctctc ctgtccctcc cctccagctg gatgctgatg atgccaaggc aacctttgat 12661 gaggaggaag gtcgggatga atacgacgaa gtggccatgc ctgtgtgagg ggcgggccca 12721 ggccctagac cctcccccac cattccacat ccccaccttc caaggaaaag cagaagttca 95
Exon 20 Anexo I - (Continuação) Apêndice 12781 tgggcacctc atggactcca ggatcctcct ggagcagcag ctgaggcccc agggctgtgg 12841 gtggggaagg aaggcgtgtc caggagacct tccacaaagg gtagcctggc ttttctggct 12901 ggggatggcc gatggggccc acattagggg gtttgttgca cagtccctcc tgttgccaca 12961 ctttcactgg ggatcccgtg ctggaagact tagatctgag ccctccctct tcccagcaca DiE20AS 13021 ggcaggggta gaagcaaagg caggaggtgg gtgagcgggt ggggtgcttg ctgtgtgacc 13081 ttgggcaagt cccttgacct ttccagccta tatttcctct tctgtaaaat gggtatattg 13141 atgataatac ccacattaca ggatggttac tgaggaccaa agatacatgt aaaatagggc 13201 tttgtaaact ccacagggac tgttctatag cagtcatcat ttgtctttga acgtacccaa 13261 ggtcacatag ctgggatttg aactgagccg tgcagctggg atttgaacca ggccttctga 13321 tttcaaggtc cgagctctgt cctctgtcag tcatgcgtcc actttccctt cccctgtgac 13381 tcctcccttc cccactctgc tcccagcccc taccttgaga ccctcttctc tgggcccaga 13441 gagaggcgtc ctggtgagga caaggtacag gcaaggatga tccagggatt gggcctggga 13501 ctcaggcctc ctaagtgttt ggttcctccc tccaaacact cattagttca ctcattcatt 13561 cattccacaa acatttactg agggccccgg aatcagtgga ctccgagggg actgagacaa 13621 gccctgccct ggggtggggg tggggggcaa ggtacagttg attctacatt tggataggga 13681 gtgggggagg gtgggaaggt aggggcggga gagtgagggg gtttgtaatt tattaattgc 13741 gtattttcta agagttttca acatagtttg gcttcacaca caacttcagg cccctcattt 13801 gagagccatt tcctcaactc catctaaact gaatcttggg gagaacccag atctgaccaa 13861 ttggggtagg agacagcagg ctctccaaga acatgggcaa atttattttt ttataaaaca 13921 aaaagataaa aagagttgaa agacgtgaaa gtggtgagag atggaggaaa cagaatcagg 13981 aagtggtaga aaagagagga ggtggctggg cgcagtggct cacgtttgta atcccagcac 14041 tttgggaggc caagttgggc ggatcatttg aggtcaggag tttgagacca gcctggccaa 14101 catggtgaaa ccccgttact actaaaaata caaaaattag ctgggtgtct cgtggcaggc 14161 acctgtaatc ccagctactt agaaggctga ggcaagagaa tcacctgaac ccaggaggtg 14221 gaggttgcag tgagccaaga ttgcaccact gcactccagc ctgggcaaca gagcaagacc 14281 ctgtctcaaa aaaaaaaaaa aaaaaaaaaa aaaacggaag gaatcatcag ccttgggggc 14341 cacagactca acatgtgtgt gtggtggggt tccagcccaa catagagtaa cattatttgt 14401 acctcccagg ctagctcagt ccatgggagg ctctcctgtc cctgaaagct gacacccacc 14461 tttcaccact tcgcccatgc tacagttcag tttcctcgtc tgtaaaatgg ggatgataat 14521 ggtacctacc ttgcagtgtt gttataagga ttaaaggaga cagtgcaaga aaaggccttg 14581 gttggtgaag agcccaacct cggaggggag ctgctgggat cctccttatc ttgactggga 14641 tgtccctgtc tccccctccc cttgctcctt gaacatggcc aaggaaagtg aaaaacaaaa 14701 attattcact ctgctagcac ccttcccctt gatgcctggg aataggtttt gccaataaac 14761 gtatctgtgt tggagtggct gccttgcctg aattc 96
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ESTUDO DO POLIMORFISMO DO SISTEMA S
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Agradeço especialmente às “meni
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Agradeço algumas pessoas pela imen
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Agradecimento ao meu marido: com qu
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Resumo Estudo do Polimorfismo do Si
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SUMMARY
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Summary studied. The DI A allele ha
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5.7.2- PCR exon 4 38 5.8- Reação
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3- Introdução Introdução Até 1
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Introdução Figura 2: Representaç
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Introdução tetrâmeros Rh e podem
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Introdução (ZELINSKI, 1993). Info
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Introdução asiáticos (chineses e
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Ocorrência do genótipo Wr a Wr a
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Introdução por polimorfismos úni
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Introdução Desta forma foram incl
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Exon 11 Exon 12 Exon 13 Exon 14 Int
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Exon 19 Exon 20 Introdução 803 At
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OBJETIVOS
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MATERIAL E MÉTODOS
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Material e Métodos Os candidatos f
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5.4- Fenotipagem Eritrocitária Mat
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Colorado, USA), de acordo com a esp
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Material e Métodos Em seguida a re
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14 50 54 70 86 139 27 74 127 40 101
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Material e Métodos Figura 19: Foto
Page 53 and 54: Material e Métodos condições: 1,
Page 55 and 56: Material e Métodos polimorfismo a
Page 57 and 58: RESULTADOS
Page 59 and 60: Resultados Figura 25: Eletroferogra
Page 61 and 62: Resultados amostras (THE HUMAN...).
Page 63 and 64: Resultados Figura 31: Eletroferogra
Page 65 and 66: Resultados Figura 35: Representaç
Page 67 and 68: Resultados Tabela 13: Relação das
Page 69 and 70: Resultados reconhece o sítio de re
Page 71 and 72: Resultados Tabela 15: Relação das
Page 73 and 74: Posição 1.314 do gene (exon 12):
Page 75 and 76: 7- Discussão Discussão Este estud
Page 77 and 78: Discussão As demais populações e
Page 79 and 80: Discussão que o índice de alosens
Page 81 and 82: Discussão se faz necessária quand
Page 83 and 84: Discussão em 2002 (1,2%) (tabela 2
Page 85 and 86: Discussão (BALEOTTI, 2002). Avalia
Page 87 and 88: Discussão Figura 41: Tronco Macro-
Page 89 and 90: Discussão estudas. Nosso resultado
Page 91 and 92: 8.Conclusões Conclusões O gene qu
Page 93 and 94: REFERÊNCIAS BIBLIOGRÁFICAS
Page 95 and 96: Newborn Attribulatable to Anti-Wr a
Page 97 and 98: http://www.socioambiental.org/websi
Page 99 and 100: APÊNDICE
Page 101 and 102: Exon 2 Exon 3 Exon 4 Exon 5 Exon 6
Page 103: Exon 14 Exon 15 Exon 16 Anexo I - (
Page 107 and 108: Anexo II FUNDAÇÃO HEMOCENTRO DE R
Page 109 and 110: Anexo III Apêndice Alinhamento das
Page 111 and 112: Anexo III (Continuação) Apêndice
Page 117 and 118: Anexo III (continuação) Apêndice
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Anexo III (Continuação) Apêndice
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Anexo IV Apêndice Relação das am
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Anexo IV Relação das amostras que
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Estudo do polimorfismo do sistema sangüíneo Diego em

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