Estudo do polimorfismo do sistema sangüíneo Diego em

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Exon 7 Exon 8 Exon 9 Exon 10 Exon 11 Exon 12 Exon 13 Anexo I - (Continuação) Apêndice 3061 ggaggctctg attctccttc catcccaggg tctctctggt ctggtctggg aagtcccaga 3121 tgtctaccct tgatgtctac cccagtccct tgatgttcgc ccttgatccc tcccaccact 3181 gatagctcag cctgaacagc ttactcagcc catgtcccct gtacgtcctg cagccacgct 3241 ggagagctgg aggccctggg gggtgtgaag cctgcagtcc tgacacgctc tggggatcct 3301 tcacagcctc tgctccccca acactcctca ctggagacac agctcttctg tgagcaggtg 3361 agtcagttac agggtcaggg tcagggtcag ggtggggcaa ggagagagct ttctcaaacg 3421 ccagcagggg tcctgaaaaa ccgaccacaa gcaggggacc agaagcactc ctgtgcccgg 3481 ggggtgccag gagagttggg aagcgagaat gggaagggga ggatgctcag gtctcccagg 3541 tcagactggc ccgaggactc ccgtcaccac cctccttccc acagggagat gggggcacag 3601 aagggcactc accatctgga attctggaaa agattccccc ggattcagag gccacgttgg 3661 tgctcgtggg taagaacagg catctctacc ctcagccctc cacacccctc gtgtcttggc 3721 tcagcctgtc tttccctcag cctggacccc cgcagcccca tctcttcagc acacccaccc 3781 tgggctcctc ggcctggcct gatcttctca tcccctaaca ggccgcgccg acttcctgga 3841 gcagccggtg ctgggcttcg tgaggctgca ggaggcagcg gagctggagg cggtggagct 3901 gccggtgcct atacgcttcc tctttgtgtt gctgggacct gaggcccccc acatcgatta 3961 cacccagctt ggccgggctg ctgccaccct catgtcagag agggtaagga agggccggag 4021 agggctgggc tggggaagct tgggacctgc atggtggctg aggcctgctc tccaacctgg 4081 cctgggttac tttaggttca ctggtttgat ccattcagcc taatccagct gctgctatcc 4141 tacctggcgg gggcagtcgg ggcgtgcagt ctggccctgc tctggcctgg tctcgtctgg 4201 ttggctttgg cctggcttag tctagtccag ctcactatga cctggttcca cctggtcagg 4261 tcaggcttgg tcgggcctag tcagctctag tttcatctag tctgcttaac ccaccaggtt 4321 agctttagtt ccttctcctt tccccaccca gggcccccgt ctgtctcccc agagctcttt 4381 cttaatgggg agagtagggg tgggagcggg caggaaaagc agttcctggc aatgggagct 4441 gctgcctgag ggggttctgg ttcctggagg ccctggctgt ggactcctgg gtcctttccc 4501 tccgcaggtc cgggcagctc tgaccctgtc tcctgcccct aggtgttccg catagatgcc 4561 tacatggctc agagccgagg ggagctgctg cactccctag agggcttcct ggactgcagc 4621 ctagtgctgc ctcccaccga tgccccctcc gagcaggcac tgctcagtct ggtgcctgtg 4681 cagagggagc tacttcgaag gcgctatcag DiI10Stccagccctg ccaagccaga ctccagcttc 4741 tacaagggcc taggtacctg cccttcccag ctgaggcttt ctgagccccc accgaccccc 4801 agacacctca gcgtagcctc tgtctcccat cacatgttcc cctctcatcg ctccctttgc 4861 ccacatcttc cagacttctt cgccgcccca ttcccatcag acaatctccc ctacacccct 4921 ccagtccctt tcccctcacc tcctccagct actccctctg accatgctct tatcctccac 4981 cacagactta aatgggggcc cagatgaccc tctgcagcag acaggccagc tcttcggggg 5041 cctggtgcgt gatatccggc gccgctaccc ctattacctg agtgacatca cagatgcatt DiEX11S 5101 cagcccccag gtcctggctg ccgtcatctt catctacttt gctgcactgt cacccgccat 5161 caccttcggc ggcctcctgg gtcagtgcca atacctgtct ccctaactct tgcctctgac 5221 cctctggcct ctgtctcagc cccaacttct gactctgtct gaccctccag acccctgcat 5281 cagcctgacc atgacttgct ctatgggctc ctggaaatga tctcctgacc ttgatcccac 5341 tgtgacctct acccacagga gaaaagaccc ggaaccagat gggagtgtcg gagctgctga DiE11AS 5401 tctccactgc DiEX12S agtgcagggc attctcttcg ccctgctggg ggctcagccc ctgcttgtgg 5461 tcggcttctc aggacccctg ctggtgtttg aggaagcctt cttctcggta gttctgtcct 5521 tgccccactg accctgtcat gtcccccgca ccacctgcct gatgcatggt gccttgcccc 5581 aaggcccttt gactgaccgg cttcttctga ccaccctgca gttctgcgag accaacggtc 5641 tagagtacat cgtgggccgc gtgtggatcg gcttctggct catcctgctg gtggtgttgg 5701 tggtggcctt cgagggtagc ttcctggtcc gcttcatctc DiE12ASccgctatacc caggagatct 5761 tctccttcct catttccctc atcttcatct atgagacttt ctccaagctg atcaaggtgg 5821 gggcctatgg ggatgttaat gcacacggga ggttgtgtat aagactgaga cagtggccgg 5881 gtgcgagggc tcaggcctgt aatctcagaa ctttgggagg ccgaggcagg tggatcaccc 5941 gagatcagga gttcaagacc agcctgacca acattgtgaa actccatctc tattaaaaat 6001 acaaaaatca gccagatgtg gtggcatgca tctgtaatcc cagctactca ggaggctgag 6061 gcaggagaac tgcttgaacc cgggaggtgg DiE13AS aggttgcagt gagccgagat tgtgccacta 6121 cactccagcc tgggcaacag agcaagaatc cgtctcaaaa aaaaaaaaaa aagactgagc 6181 cattgtgtcc tcgcacatgt gtctgtgcag catttgtgtg tctttgcttt cacctgtgta 6241 cataaccatg tgactgtgca catgactgtg acaacatatg tgtctgtgtc aacatatgtg 93
Exon 14 Exon 15 Exon 16 Anexo I - (Continuação) Apêndice 6301 tctatgtctg tgtagtcaca catctgcaca caggactgtg cagctgtgtt gactgtgacc 6361 acatatgtgt tggcacctgc tgcctgtggc cagcactctt gctgaggcat gagagggtgc 6421 ctctgtgtgc acatgtccag ttcatgacca cacatgacgt gagcaatggg taggtatggg 6481 catgacactg agcatgtgaa agtacatgca cagtgagagc tggggggact ctggagcctc 6541 tgaagtcccc cactccctcc cctgtgcttg tcctcaagag ctgcagggct tgtggatgga 6601 gtgcccagcc accttggctt ggctcccatc atcttaagct ctgcttgcct ttgtacctgc 6661 attttatatg ggagaagggt gagccctatg tgttgtcccc tgattctgtt ggatggaggg 6721 tctttctgtg tccaggtctt ttaacccctt gttagaaact tccctgaccc ttgtttcctg 6781 acctccagcc ttctcatgtc tcccaggtcc tgggtgtaca tgtgtgctcg cacacacacc 6841 cattgccttc catggccctc cctgcctaaa cctaggctgt cttcattcct gaaaaccaag 6901 ctgtagaatc ccatttatcc tctgtttcca tgtcaattga ggaagaagat agatggatgg 6961 atggatgaat agatggataa tggatggacg gatggatgga tggatggatg gttggatgga 7021 tggatgattg atgaatgagt gggtggatga tggacggatg aatggatgga taaatgggtt DiEX13S 7081 gatagatgat aggtaggtgg atggatgggt agatggataa tgaatggatg gatgaataat 7141 caatggatga tcaatggata atggatagat gaatgatgga tgggtggata aatggatgga 7201 tgatgaatta atagatgact ggatgaatgg atagtagtgc tgggtagagt tagctggtgg 7261 tattttccag cccaagccaa agctgggaga gaacagaatg ccttggtttt ctgctgcaga 7321 tcttccagga ccacccacta cagaagactt ataactacaa cgtgttgatg gtgcccaaac 7381 ctcagggccc cctgcccaac acagccctcc tctcccttgt gctcatggcc ggtaccttct 7441 tctttgccat gatgctgcgc aagttcaaga acagctccta tttccctggc aaggtcagca 7501 taccctcctc gcctgtcctt gccaacactg ccctatccca gcccaaagcc cccccgctcc 7561 aaattcctca gtgcccttag cttcccttct ttttcagatt cccattccca attccaacct 7621 cccctggccc tatccttacc tagggtttca ctctatgggc DiEX14S aagcccctcc cactttcatg 7681 gcatgcccag cctgagaact gctgggggga DiE14AS agaagaaagg aggcagacat agctgaggtg 7741 caggacaggg cattagatgc tgatggatcc cctggaagag cagggccccg tgggtgaata 7801 tagggcagga ggaaggcagg aggtggggag tgactgggca ctgaccactg gtcctttcac 7861 ctgcccccag ctgcgtcggg tcatcgggga cttcggggtc cccatctcca tcctgatcat 7921 ggtcctggtg gatttcttca ttcaggatac ctacacccag gtgacccctc ctcccagccc 7981 tcagaccatg atacccccca ggtcctcatt tccacccccc agcatgcccc ttccctagaa 8041 tagctttccc caacctgaag ccagctggga ctacctctac cccatccctg cctcctctgg 8101 gagtcccatg tgcttctcac agccccagag gacccctgtg gccaaagcaa ggtgctgggc 8161 caggttgcca ccttcattcc DiE15ASttaacccaga gcagtaggaa gaagtattct ttgagccagg 8221 cagatctagg DiEX15S gatgaatccc agctctgcca tcaaatagct gtgtgatgtg gggcaagcta 8281 cataacctct ctgattctct ttcctcatct ttaaagtggg gaacgagcag atggatgtaa 8341 agggattagt gtaatgatta ggtacagagc aagtgctcaa tataagtaag ttcccaagtg 8401 cctccaacct aactgcccga cccctaggac cctggctcct cctgctccca cccttcccca 8461 tcctcctgcc cttggcattc ttacctgcta ccccctccca cagaaactct cggtgcctga 8521 tggcttcaag gtgtccaact cctcagcccg gggctgggtc atccacccac tgggcttgcg 8581 ttccgagttt cccatctgga tgatgtttgc ctccgccctg cctgctctgc tggtcttcat 8641 cctcatattc ctggagtctc agatcaccac gtgagagaca gggtgggggc tgggcctggg 8701 agggtgggag gcagggacct ttccctggca ttctcccagg caggcaagag acccaggact 8761 gcaagctagt ccctgcgggt gtgtggccct cccgactagc aggaatagtg aaaagccagg 8821 ggcagttcca ggttttgtgg gaccagaagt tgtataattt ggagagctct ttttaagaaa 8881 aaaaaaacgt ggccgggcgc ggtggctcac gcccgtaatc ccaacagttt gagaggccga 8941 ggcgggcaga tcacctgagg DiE16AStcaggagttc gagaccagct tggctaacat ggtgaagccc 9001 tgtttctact aaaaatacaa aaaattaggc gggcatggtg gcacgagcct gtaatctcag 9061 ctacttggga ggctgaggca ggagaatcgc ttgaacccgg gagggccgga ggttgcagtg 9121 agccgagatc tcgccattgc actccaactt gggcaacgag agtgaaactc catctcaaaa 9181 aaaaaaaaaa aaaaaaagcg ccaggtgagg tggctcacat ctgtaatcct agcactttgg 9241 gaagccaaga cggggggatc acgaggtcag tagtttgaga ccagcctgac taatatggtg 9301 aaaccccgtt tctactaaaa atacaaaaat taaccgagtg tggtggcatg tgcccgtagt 9361 cccagctact tgggaggctg aagcaggaaa atcacttgaa cccgggaggc agaggttgca 9421 gtgagccaaa atcgcaccac tgcactccag cctgggcgac agagcaagac tctaggaaaa 9481 aaaagaaaaa gaaaaaaaaa tgcaaaatga caactataaa attaggtgct ggatcttgga 94
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ESTUDO DO POLIMORFISMO DO SISTEMA S
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Agradeço especialmente às “meni
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Agradeço algumas pessoas pela imen
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Agradecimento ao meu marido: com qu
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Resumo Estudo do Polimorfismo do Si
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SUMMARY
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Summary studied. The DI A allele ha
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5.7.2- PCR exon 4 38 5.8- Reação
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3- Introdução Introdução Até 1
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Introdução Figura 2: Representaç
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Introdução tetrâmeros Rh e podem
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Introdução (ZELINSKI, 1993). Info
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Introdução asiáticos (chineses e
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Ocorrência do genótipo Wr a Wr a
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Introdução por polimorfismos úni
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Introdução Desta forma foram incl
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Exon 11 Exon 12 Exon 13 Exon 14 Int
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Exon 19 Exon 20 Introdução 803 At
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OBJETIVOS
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MATERIAL E MÉTODOS
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Material e Métodos Os candidatos f
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5.4- Fenotipagem Eritrocitária Mat
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Colorado, USA), de acordo com a esp
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Material e Métodos Em seguida a re
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14 50 54 70 86 139 27 74 127 40 101
Page 51 and 52: Material e Métodos Figura 19: Foto
Page 53 and 54: Material e Métodos condições: 1,
Page 55 and 56: Material e Métodos polimorfismo a
Page 57 and 58: RESULTADOS
Page 59 and 60: Resultados Figura 25: Eletroferogra
Page 61 and 62: Resultados amostras (THE HUMAN...).
Page 63 and 64: Resultados Figura 31: Eletroferogra
Page 65 and 66: Resultados Figura 35: Representaç
Page 67 and 68: Resultados Tabela 13: Relação das
Page 69 and 70: Resultados reconhece o sítio de re
Page 71 and 72: Resultados Tabela 15: Relação das
Page 73 and 74: Posição 1.314 do gene (exon 12):
Page 75 and 76: 7- Discussão Discussão Este estud
Page 77 and 78: Discussão As demais populações e
Page 79 and 80: Discussão que o índice de alosens
Page 81 and 82: Discussão se faz necessária quand
Page 83 and 84: Discussão em 2002 (1,2%) (tabela 2
Page 85 and 86: Discussão (BALEOTTI, 2002). Avalia
Page 87 and 88: Discussão Figura 41: Tronco Macro-
Page 89 and 90: Discussão estudas. Nosso resultado
Page 91 and 92: 8.Conclusões Conclusões O gene qu
Page 93 and 94: REFERÊNCIAS BIBLIOGRÁFICAS
Page 95 and 96: Newborn Attribulatable to Anti-Wr a
Page 97 and 98: http://www.socioambiental.org/websi
Page 99 and 100: APÊNDICE
Page 101: Exon 2 Exon 3 Exon 4 Exon 5 Exon 6
Page 105 and 106: Exon 20 Anexo I - (Continuação) A
Page 107 and 108: Anexo II FUNDAÇÃO HEMOCENTRO DE R
Page 109 and 110: Anexo III Apêndice Alinhamento das
Page 111 and 112: Anexo III (Continuação) Apêndice
Page 117 and 118: Anexo III (continuação) Apêndice
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Anexo III (Continuação) Apêndice
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Anexo IV Apêndice Relação das am
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Anexo IV Relação das amostras que
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Estudo do polimorfismo do sistema sangüíneo Diego em

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