Introduction to Soil Chemistry
Introduction to Soil Chemistry
Introduction to Soil Chemistry
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140 extraction<br />
serious interferences during analytical procedures. The standard method of<br />
separation is filtration, which can be accomplished using standard labora<strong>to</strong>ry<br />
paper filter papers. Often labora<strong>to</strong>ry personnel will crease filter paper with<br />
such force that small tears or holes are <strong>to</strong>rn in the paper, allowing soil <strong>to</strong> pass<br />
though during filtration. In other situations the porosity of the paper may be<br />
sufficient <strong>to</strong> allow clay particles and organic matter <strong>to</strong> pass through the paper.<br />
In this case refiltering through the same paper will sometimes be sufficient <strong>to</strong><br />
remove all particles. Sometimes two layers of the same filter paper will be sufficient.<br />
Some combination of these approaches used <strong>to</strong>gether should remove<br />
all particles.<br />
Sometimes, however, none of the above mentioned procedures will be<br />
sufficient, and other filtering methods may be required. Small filtering disks<br />
that fit on a plastic syringe and have porosities of 0.22 or 0.45mm will<br />
effectively remove particles from soil extract. These disks clog rapidly with<br />
soil, and so it is essential that the suspension be filtered through filter<br />
paper before using filtering disk, particularly if significant quantities of filtrate<br />
are needed. Either the suspension <strong>to</strong> be filtered can be pulled in<strong>to</strong> the<br />
syringe, the filter disk added, and the suspension expressed out of the syringe;<br />
or the disk can be put on the syringe, the plunger removed, suspension<br />
poured in<strong>to</strong> the syringe, the plunger inserted, and the suspension expressed,<br />
thereby filtering it.<br />
An alternate procedure is <strong>to</strong> use a centrifuge <strong>to</strong> remove suspended material<br />
from soil extracts. Centrifugation is very effective in removing small particles;<br />
however, caution must be exercised when removing centrifuged<br />
suspensions from the centrifuge and centrifuge tube because any motion may<br />
resuspend particles, thus undoing the centrifugation process. The supernatant,<br />
when carefully removed, can be used directly for analysis. In many cases this<br />
is faster than filtration, especially when a large quantity of solution free of suspended<br />
material is required.<br />
7.5.2. Sorption Cleanup Methods<br />
There are a myriad of sorption cleanup procedures available. Some are simple,<br />
requiring little sample preparation and manipulation; others are more<br />
complex. Common cleanup materials along with their advantages and disadvantages<br />
are listed in Table 7.3. These sorption materials can be used in glass<br />
columns similar <strong>to</strong> chroma<strong>to</strong>graphic columns, or they may be purchased as<br />
prepared columns, termed solid-phase extraction columns, the use of which is<br />
simply called solid-phase extraction (SPE) (see discussion above).<br />
The basic method involves passing the extract through a column containing<br />
an appropriate solid sorbing material. One of two cleanup processes can<br />
occur.The component of interest can be sorbed and thus separated from other<br />
components. It is later released by extraction and analyzed. The other process<br />
involves the component passing through the column while unwanted components<br />
are retained on the column. Both of these can be effective cleanup