Introduction to Soil Chemistry
Introduction to Soil Chemistry
Introduction to Soil Chemistry
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186 chroma<strong>to</strong>graphy<br />
Retention times can be used for “identification” of eluting compounds in<br />
the same way as with CG [4–6].<br />
9.3.5. High-Performance Liquid Chroma<strong>to</strong>graphy Applied <strong>to</strong> <strong>Soil</strong><br />
In soil analysis HPLC is used much like GC in that soil is extracted and the<br />
extract, after suitable cleanup and concentration, is analyzed. One major difference<br />
between them is that HPLC does not require that components be in<br />
the gaseous phase. They must, however, be soluble in an elutant that is compatible<br />
with the column and detec<strong>to</strong>r being used. Another difference is that<br />
both a syringe and an injec<strong>to</strong>r are used <strong>to</strong> move the sample in<strong>to</strong> the elutant<br />
and on<strong>to</strong> the column. Detection is commonly by UV absorption, although both<br />
refractive index and conductivity are also commonly used. Conductivity or<br />
other electrical detection methods are used when analysis of ionic species in<br />
soil is carried out [2,6,7].<br />
9.4. THIN-LAYER CHROMATOGRAPHY<br />
Thin-layer chroma<strong>to</strong>graphy (TLC) is carried out on a thin layer of adsorbant<br />
on a glass or plastic support (other supports have been used). It has sometimes<br />
been referred <strong>to</strong> as planar chroma<strong>to</strong>graphy since the separation occurs in a<br />
plane. Paper chroma<strong>to</strong>graphy, which is carried out using a piece of paper,<br />
usually filter paper, is very similar <strong>to</strong> thin-layer chroma<strong>to</strong>graphy and will not<br />
be covered here.<br />
9.4.1. Sample Application<br />
In thin-layer chroma<strong>to</strong>graphy a 10-mL or larger syringe is often used <strong>to</strong> place<br />
sample spots on the thin layer before development. Alternatively, a glass capillary<br />
tube or Pasteur pipette may be heated in a burner and pulled <strong>to</strong> obtain<br />
a fine capillary suitable for spotting (see Figure 9.3).<br />
9.4.2. Mobile Phases<br />
Development of the thin-layer chroma<strong>to</strong>gram is accomplished by placing a<br />
small amount of elutant in the bot<strong>to</strong>m of a suitable container then placing the<br />
spotted thin layer in the container, sealing it, and allowing the elutant <strong>to</strong> ascend<br />
the layer through capillary action.<br />
9.4.3. Stationary Phases<br />
A thin layer of adsorbant is applied <strong>to</strong> a support that may be a sheet of glass,<br />
metal, or plastic (Figure 9.4, D). Adsorbants are typically alumina, silica gel,<br />
or cellulose and may be mixed with gypsum <strong>to</strong> aid in adhering <strong>to</strong> the support.