Introduction to Soil Chemistry
Introduction to Soil Chemistry
Introduction to Soil Chemistry
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thin-layer chroma<strong>to</strong>graphy 187<br />
They may also include a fluorescent indica<strong>to</strong>r that aids in visualization once<br />
the plate is developed.These adsorbants may also have hydrocarbons attached<br />
<strong>to</strong> them such that reverse-phase TLC can be carried out.<br />
9.4.4. Detection<br />
Caution: Spraying thin layer plates with visualization reagents should<br />
always be done in a functioning hood. These reagents may be caustic<br />
and <strong>to</strong>xic, as is the case with sulfuric acid.<br />
Thin-layer plates, once developed and dried, are sprayed with a visualization<br />
reagent that allows the detection of the separated components. The visualization<br />
reagent produces a colored spot where each component of the mixture is<br />
on the plate. For example, a 0.1% solution of ninhydrin (1,2,3-indantrione<br />
monohydrate) in ace<strong>to</strong>ne can be sprayed on a plate that has separated amino<br />
acids on it. The amino acids will show up as blue <strong>to</strong> brown spots on the plate.<br />
Sulfuric acid can be sprayed on a silica or alumina plate and heated <strong>to</strong> show<br />
the position of organic compounds as charred spots (this reagent cannot be<br />
used on cellulose plates since the whole plate will turn black). Reducing compounds<br />
can be visualized by using a solution of ammonical silver nitrate, which<br />
will produce black spots where the silver is reduced by the reducing compound.<br />
This reagent is particularly sensitive for reducing sugars. There are a<br />
whole host of different visualizing reagent that can be found in the book by<br />
Hellmut et al. [8].<br />
A method for detecting compounds that have UV absorbence is the use of<br />
thin-layer plates that contain a fluorescence dye or indica<strong>to</strong>r. Plates are developed<br />
and then placed under an ultraviolet light. Compounds on the plate<br />
may show up as bright or dark spots depending on their interaction with the<br />
ultraviolet light and the fluorescence produced by the fluorescence indica<strong>to</strong>r<br />
[8,9].<br />
9.4.5. <strong>Soil</strong> Thin Layer<br />
A variation on thin-layer chroma<strong>to</strong>graphy is the use of soil thin layers <strong>to</strong> investigate<br />
the movement and degradation of organic compounds in soil. A soil<br />
is sieved using a number 200 or smaller sieve, and this soil is used <strong>to</strong> produce<br />
a suspension of soil in distilled water that is then spread on a glass sheet<br />
<strong>to</strong> produce a soil thin layer. An organic component, often a herbicide, is<br />
spotted on the dried sheet, which is developed using deionized water. The<br />
movement and degradation of the organic compound under these conditions<br />
can then be related <strong>to</strong> its expected movement and degradation in soil in the<br />
field [10].