Introduction to Soil Chemistry
Introduction to Soil Chemistry
Introduction to Soil Chemistry
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158 spectroscopy<br />
placed in the sample cell, which is placed in the sample compartment of the<br />
instrument and a spectrum obtained. Many compounds have large molar<br />
absorptivities, 1 and thus only small amounts are needed <strong>to</strong> obtain a spectrum.<br />
Most UV–Vis spectropho<strong>to</strong>meters function best between 0 and 1 absorbence.<br />
Often a sample will have an absorbence above 1, as is the case illustrated in<br />
Figure 8.6. In these cases it is usually most useful <strong>to</strong> dilute the sample by a<br />
fac<strong>to</strong>r of 10 <strong>to</strong> bring the absorption below 1 before using the absorbence data.<br />
8.7. THE VISIBLE REGION<br />
The visible region of the spectrum, with wavelengths 400nm (violet) and<br />
900nm, is used extensively in soil analysis in the colorimetric determination of<br />
components extracted from soil (see Section 8.8). Once extraction is complete<br />
and the extract has been filtered and otherwise cleaned as needed, it is analyzed<br />
for the components of interest by treating it with a reagent <strong>to</strong> produce a colored<br />
product. The amount of color is directly related <strong>to</strong> the amount of component<br />
present.<br />
An excellent example of this type of analysis involves the determination<br />
of phosphate in soil extracts. <strong>Soil</strong> is extracted with an appropriate extractant<br />
and added <strong>to</strong> a solution of acid molybdate, with which the phosphate reacts<br />
<strong>to</strong> produce a purple or blue, phosphomolybdate solution. Standard phosphate<br />
solutions are prepared and reacted with acid molybdate, and the intensity of<br />
the phosphomolybdate produced is measured. A standard of calibration curve<br />
is prepared from which the intensity of the color is directly related <strong>to</strong> the<br />
concentration of phosphate in the extract.<br />
For this type of analysis <strong>to</strong> be accurate, three characteristics must exist:<br />
1. The soil extract must not contain any components that absorb light at<br />
the same wavelength as the phosphomolybdate; this includes suspended<br />
material that will refract light. Refracted light does not get in<strong>to</strong> the detec<strong>to</strong>r<br />
and so is recorded as being absorbed by the sample, thus giving an<br />
inaccurate result.<br />
2. The color producing reagent must not react with any other commonly<br />
occurring component in the extract <strong>to</strong> form a similarly colored product.<br />
3. The soil must not contain any compound that inhibits or interferes with<br />
the production of the colored compound.<br />
As described in previous chapters, soil contains many different inorganic<br />
and organic elements, ions, and compounds plus both inorganic and organic<br />
colloids. Thus it cannot be assumed that the soil being investigated does not<br />
contain any of the types of interference mentioned above. Some soils high in<br />
1 Absorbtivity is defined as A =ebC, where A = absorbance, e=molar absorption coefficient (liter<br />
mol -1 cm -1 ), b = pathlength of radiation through sample (cm), and C = molar concentration.