Introduction to Soil Chemistry
Introduction to Soil Chemistry
Introduction to Soil Chemistry
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analysis 65<br />
3.7.1. Analysis for Animals and Plants<br />
Determination of the number of animals in a cubic meter of soil is usually<br />
done by simple isolation and counting. Plants are usually counted as the<br />
number per square meter, hectare, or acre. Determination of root numbers or<br />
mass is extremely difficult and is commonly done by assuming that there is a<br />
certain mass of roots associated with a plant’s <strong>to</strong>p, that is, the ratio of roots <strong>to</strong><br />
<strong>to</strong>p masses. Determination of microorganisms is usually done using standard<br />
microbiological techniques such as dilution plate counts. Because of the<br />
extreme diversity of soil microorganism, it is impossible <strong>to</strong> determine all the<br />
organisms present at one time by any one technique. In some cases direct<br />
microscopic observation, as was done <strong>to</strong> produce Figure 3.6, is used <strong>to</strong> estimate<br />
the numbers of microorganisms present [2–4].<br />
3.7.2. Determination of <strong>Soil</strong> Organic Matter<br />
The analysis of soil for organic matter 4 is straightforward, involving oxidizing<br />
it <strong>to</strong> carbon dioxide and water. Oxidation can be accomplished in a number<br />
of different ways, such as by applying air at high temperature, pure oxygen, or<br />
chemical oxidation using various common chemical oxidizing agents such as<br />
hydrogen peroxide, permanganate, and dichromate followed by titration. Of<br />
these, oxidation by hot dichromate followed by titration of unreduced dichromate<br />
is most commonly used.<br />
The real problem is the complete oxidation of soil organic matter. As noted<br />
previously, this can be associated with mineral surfaces or in pores <strong>to</strong> which<br />
there is difficult access. For these reasons, simple oxidation will not be sufficient,<br />
and drastic oxidation procedures will be necessary.<br />
3.7.2.1 Direct Oxidation Using High Temperature and Atmospheric Air<br />
The simplest and least expensive method of determining the organic matter<br />
in soil is high-temperature oxidation using air. In this case a weighed soil<br />
sample in a crucible is placed in an oven and dried. Subsequently, after a prescribed<br />
time at an elevated temperature, it is again removed and weighed, and<br />
the difference is taken as the amount of organic matter. This procedure is<br />
seemingly simple, straightforward, and easy <strong>to</strong> perform and does not involve<br />
the expense of waste disposal. While oxidation of organic matter with a consequent<br />
loss of weight does occur, so do other reactions, which lead <strong>to</strong> a loss<br />
of weight. One such reaction is the loss of waters of hydration from soil minerals.<br />
Another is the decomposition of soil minerals. For instance, carbonates,<br />
which are very common soil minerals, decompose at high temperature <strong>to</strong> form<br />
4 Specific directions for determination of soil organic matter are given in the bibliographic<br />
references.