Introduction to Soil Chemistry
Introduction to Soil Chemistry
Introduction to Soil Chemistry
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
192 chroma<strong>to</strong>graphy<br />
sufficient. True identification occurs when HPLC and GC are coupled <strong>to</strong> a<br />
spectropho<strong>to</strong>metic method such as MS and/or UV–Vis.<br />
PROBLEMS<br />
9.1. Explain how all chroma<strong>to</strong>graphic methods are similar.<br />
9.2. Explain how the basic chroma<strong>to</strong>graphic methods are different.<br />
9.3. What physical characteristics must components have <strong>to</strong> be separated by<br />
gas chroma<strong>to</strong>graphy?<br />
9.4. What do the terms Rf and Rt refer <strong>to</strong>? Explain how they are used in<br />
determining what components are likely <strong>to</strong> be present in a mixture.<br />
9.5. Describe detection methods used in GC, HPLC, and TLC.<br />
9.6. What general characteristic must a component have <strong>to</strong> be separated by<br />
electrophoresis?<br />
9.7. How are soil thin-layer plates used in environmental investigations?<br />
9.8. Explain why it is advantageous <strong>to</strong> have separated compounds exiting a<br />
GC or HPLC column analyzed by MS.<br />
9.9. What is the difference between the mobile phase and the stationary<br />
phase in chroma<strong>to</strong>graphy?<br />
9.10. The chroma<strong>to</strong>grams from two different injections have the following<br />
components (peaks) with the indicated retention times. Injection 1: peak<br />
1, Rt = 0.55; peak 2, Rt = 1.25; peak 3, Rt = 2.44; peak 4, Rt = 5.65. Injection<br />
2: peak 1, Rt = 0.22; peak 2, Rt = 1.00; peak 3, Rt = 5.65; peak 4,<br />
Rt = 6.74. Which of the peaks in the two chroma<strong>to</strong>grams is likely <strong>to</strong> be<br />
the same compound?<br />
BIBLIOGRAPHY<br />
Ardrey RE. Liquid Chroma<strong>to</strong>graphy–Mass Spectrometry: An <strong>Introduction</strong>. New York:<br />
Wiley, 2003.<br />
Grob RL, Barry EF. Modern Practice of Gas Chroma<strong>to</strong>graphy. New York: Wiley-<br />
Interscience, 1995.<br />
Niessen WMA (ed.). Current Practice of Gas Chroma<strong>to</strong>graphy–Mass Spectroscopy.<br />
New York: Marcel Dekker, 2001.<br />
Snyder LR, Kirkland JJ, Glajch JL. Practical HPLC Method Development, 2nd ed.<br />
New York: Wiley-Interscience, 1997.<br />
Tabatabai MA, Frankenberger WT Jr. Liquid chroma<strong>to</strong>graphy. In Methods of <strong>Soil</strong><br />
Analysis, Part 3: Chemical Methods, Bartels JM (ed.). Madison, WI: <strong>Soil</strong> Science<br />
Society of America, American Siociety of Agronomy, pp. 225–245, 1996.