Introduction to Soil Chemistry
Introduction to Soil Chemistry
Introduction to Soil Chemistry
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Mobile<br />
phase in<br />
abaybayby<br />
abaybayby<br />
abaybayby<br />
abaybayby<br />
yyy bbb aaa<br />
yyy<br />
yyy<br />
yyy<br />
bbb aaa<br />
bbb aaa<br />
bbb aaa<br />
gas chroma<strong>to</strong>graphy 177<br />
aaa<br />
aaa<br />
aaa<br />
aaa<br />
bbb<br />
bbb<br />
bbb<br />
bbb<br />
yyy bababa<br />
yyy bababa<br />
yyy bababa<br />
yyy bababa<br />
yyy<br />
yyy<br />
yyy<br />
yyy<br />
Exit <strong>to</strong><br />
detec<strong>to</strong>r<br />
Figure 9.2. Separation of a complex mixture by chroma<strong>to</strong>graphy. The <strong>to</strong>p segment shows the<br />
column just after application of mixture. As the sample progresses through the column, separation<br />
of mixture occurs as a result of the chroma<strong>to</strong>graphy. The first component exits the column<br />
and is detected, followed by the other two components.<br />
introduced in<strong>to</strong> a gas chroma<strong>to</strong>graph, must either already be a gas or be<br />
immediately turned in<strong>to</strong> a gas on injection. A complete chroma<strong>to</strong>gram is<br />
usually obtained in less than 20min, although some analysis can take much<br />
longer.<br />
9.2.1. Sample <strong>Introduction</strong><br />
For gas chroma<strong>to</strong>graphy a 1 <strong>to</strong> 0.1mL syringe, shown in Figure 9.3, which has<br />
the entire sample contained in the needle, is frequently used for the introduction<br />
of the sample in<strong>to</strong> the injection port.The needle must be of the correct<br />
type and length for the gas chroma<strong>to</strong>graph being used, or poor results will be<br />
obtained. Injection is effected through a septum made of special rubber;<br />
Teflon-backed septa are also available and must be in good condition for accurate<br />
chroma<strong>to</strong>grams <strong>to</strong> be obtained. Injection ports without septa are also<br />
available. When a new septum has been placed in the injection port, piercing<br />
it with a syringe needle will be harder, and care must be taken not <strong>to</strong> bend the<br />
needle or syringe plunger. When the needle is fully inserted, the plunger is<br />
depressed and a recording of the detec<strong>to</strong>r output is started. This is the 0 time<br />
used in calculating the retention times (R t, described below) of emerging<br />
peaks. Needle insertion and depression of the plunger must be done in one<br />
continuous motion <strong>to</strong> obtain a good chroma<strong>to</strong>gram. GCs can be fitted with<br />
au<strong>to</strong>matic samplers that take and inject the sample au<strong>to</strong>matically.