Introduction to Soil Chemistry
Introduction to Soil Chemistry
Introduction to Soil Chemistry
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color measurement: the spectropho<strong>to</strong>meter 159<br />
organic matter may have dark-colored components that are released in<strong>to</strong> the<br />
extracting solution and cause interference with the spectropho<strong>to</strong>metric<br />
analysis. Thus extracts must be analyzed <strong>to</strong> make sure that they are free of<br />
interference.<br />
8.8. COLOR MEASUREMENT: THE SPECTROPHOTOMETER<br />
Color measurement is based on the Beer–Lambert law, which can be<br />
expressed as follows:<br />
I<br />
A = log10 I<br />
In this equation A is the absorbence also called the optical density, I is the<br />
intensity of the bean of light entering the sample, and I ex is the intensity of the<br />
beam of light exiting the sample. The absorbance is proportional <strong>to</strong> both<br />
the concentration of absorbing species and the pathlength of the light through<br />
the sample. Normally standard-size sample cells are used, and so the pathlength<br />
is constant for all samples.<br />
This type of analysis involves colored solutions; thus the spectropho<strong>to</strong>meter<br />
need function only in the visible range of the spectrum. A visible light source<br />
is used <strong>to</strong> provide light that passes through the sample in a cuvette, <strong>to</strong> a grating<br />
where it is dispersed in<strong>to</strong> its respective wavelengths. The analytical wavelength,<br />
that is, the wavelength absorbed by the colored compound, is isolated<br />
and directed <strong>to</strong> the detec<strong>to</strong>r, where the amount of light at this wavelength is<br />
measured and displayed on the readout.A diagram of a spectrometer is shown<br />
in Figure 8.8. Note that there are many different ways <strong>to</strong> design a spectropho<strong>to</strong>meter<br />
and its light path. The diagram given is simply a general representation<br />
of how a spectropho<strong>to</strong>meter works.<br />
Cuvettes used with the spectropho<strong>to</strong>meter are not simply test tubes. They<br />
are specially made tubes and are often matched such that a set of tubes will<br />
all have the same absorbence characteristics. Cuvettes should never be used<br />
as test tubes; they must be kept clean at all times, and care must be taken not<br />
Slit<br />
Light source<br />
Sample<br />
cuvette<br />
Read out<br />
0.55<br />
ex<br />
Detec<strong>to</strong>r<br />
Grating<br />
Figure 8.8. Diagram of the light path in a spectropho<strong>to</strong>meter.