Protocols - Hemorio

PRESERVATION AND CONSERVATION: The concentrate of Whole Blood Platelets contains 5.5 x 10
platelets by bag, in approximately 50 to 70 ml of plasma. The anti-clotting-preservative solution is the
same as the whole blood bag initially collected.
Platelets obtained by apheresis contain > 3.0x10 11 platelets in approximately 250 ml of plasma. The anticlotting-preservative
solution is ACD. The conservation temperature is 22 o C + or – 2 o C and in a constant
stir.
SELECTION AND PREPARATION: The platelets concentrates must be ABO-compatible whenever
possible. The negative Rh receptors must receive negative Rh platelets, mainly child-bearing potential
women, and female children.
The Whole Blood Platelets Concentrates must be transfused under the “pool” form. They are prepared by
the Hemotherapy Service and can be 4, 8 or 10 platelets concentrates, depending on the indication.
Apheresis platelets are used in an only dosage.
The response to the platelets transfusion is lesser than those expected when there are: fever, sepsis,
splenomegaly, severe hemorrhage, consumption coagulopathy, HLA alloimmunization and certain drugs
(amphotericin).
INDICATIONS: The platelets concentrate pool or apheresis platelets are indicated when there is bleeding
due to the decreased counting of circulating platelets or when there are platelets functionally abnormal.
CONTRAINDICATIONS: Do not use in Self-immune Thrombocytopenia and in Thrombotic
Thrombocytopenic Purpura.
4 – FROZEN FRESH PLASMA:
DESCRIPTION: The Frozen Fresh Plasma - PFC is obtained by centrifugation from a whole blood bag
collected. It can also be obtained by apheresis. This product must be totally frozen in up to 8 h after the
collection. The anticoagulant is the same as the whole blood bag of source. The volume is approximately
250 ml.
SPECIAL PREPARATIONS:
Quarantine Plasma – is the plasma whose serologic tests were not reagent, kept at a temperature of -20
ºC or below, and that is not used at the time of the donation. It is kept until the return of the donor for a
new donation. If the new screening tests are unchanged, the hemocomponent will be released to use. This
procedure aims at a higher transfusional safety. The quarantine plasma must not be taken for the plasma
blocked for usage, awaiting the results of the mandatory serologic screening.
Cryo-Free Plasma – is the plasma from which the cryoprecipitate was removed, in a closed system. It
must be frozen at -20 o C or below and has a validity of 5 years.
PRESERVATION AND CONSERVATION: By definition, each 1 ml of plasma contains 1 IU of each
clotting factor. The conservation temperature is at least -20 o C, but it is recommended the temperature of -
30 o C.
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