Protocols - Hemorio

LEUKOCYTARY ALKALINE PHOSPHATASE (FAL) – NORMAL SCORE = 40-90
FAL – LOW VALUES FAL – HIGH VALUES
CML
INFECTIOUS STATES
HYPERTHYROIDISM POLYCYTHEMIA VERA
FALCIFORM ANEMIA HEMOLYTIC ANEMIA
HPN
MYELOFIBROSIS
PREGNANCY
2 - IMMUNOPHENOTYPE:
IMMUNOPHENOTYPE PROFILE OF THE ACUTE LYMPHOID LEUKEMIAS:
SUBTYPE COMMON PHENOTYPE
LLA B- HLADR, CD19, CD20-/+, CD10, CD34, TDT
precursor
LLA pre-B HLADR, CD19, CD10, CD34, TdT-/+, IgMc, CD 20+/-
LLA-B HLADR, CD19, CD20, CD22, CD10-/+, CD34 -, TdT - , sIG
LLA-T HLADR-/+, CD1, CD2, cCD3, CD5, CD7, CD4/C8, CD10+/- CD34-/+, CD45, TdT
Abbreviations: (+ / -) - variable, most of times positive; (- / +) - variable, most of the times negative, (c ) -
cytoplasmic
IMMUNOPHENOTYPE PROFILE OF THE LYMPHOPROLIFERATIVE DISEASES:
LYMPHOPROLIFERATIVE B DISEASES
DISEASES COMMON PHENOTYPE
CHRONIC LYMPHOCYTIC LEUKEMIA
HLADR, CD19, CD20, CD5, CD22 (-), CD23, CD10 (-),
CD11c+/-, CD25+/, SIGM clonal
PROLYMPHOCYTE LEUKEMIA HLADR, CD19, CD20, CD5 (-), CD22, CD23 (-), CD10 (-), sIG
MANTLE CELL LYMPHOMA HLADR, CD19, CD20, CD5, CD22, CD23 (-), CD10 (-)
FOLLICULAR LYMPHOMA
HLADR, CD19, CD20, CD5 (-), CD22, CD23+/-, CD10, CD11c
(-)
MARGINAL AREA AND ASSOCIATES HLADR, CD19, CD20, CD5 (-), CD22, CD23 (-), CD10 (-),
LYMPHOMA
CD11c, CD25 (-), CD103 (-)
HAIRY CELL LEUKEMIA
HLADR, CD19, CD20, CD5 (-), CD22, CD23 (-), CD10 (-),
CD11c, CD25, CD103
Abbreviations: (+ / -) - variable, most of times positive; (- / +) - variable, most of the times negative (-)
negative; (Sig) – surface immunoglobulin; (cIG) cytoplasmic immunoglobulin.
LYMPHOPROLIFERATIVE T DISEASES
DISEASES COMMON PHENOTYPE
PROLYMPHOCYTE T LEUKEMIA CD2, CD3, CD5, CD7, CD4, CD8 (-)
BIG GRANULE LYMPHOCYTES (LGL- CD2, CD3, CD5 (-), CD7 (-), CD4 (-), CD8, CD16, CD11b,
T)
CD56 (-), CD57, CD25 (-)
BIG GRAN. LINF LEUKEMIA NK CD2, CD3 (-), CD16, CD56, CD4 (-), CD8-/+, CD56, CD57 (-),
CD25 (-)
FUNGOID MYCOSIS (SÉZARY SDR) CD2, CD3, CD5, CD7+/-, CD4, CD8 (-), CD25 (-)
LEUKEMIA / T-CELL LYMPHOMA OF CD2, CD3, CD5, CD7 (-), CD4, CD8 (-), DR, CD25 INTENSE
ADULT
CHRONIC LYMPHOID LEUKEMIA OF CD2, CD3, CD5, CD7 (-), CD4, CD8 (-)
T-CELL
Abbreviations: (+ / -) - variable, most of times positive; (- / +) - variable, most of the times negative (-)
negative; (Sig) – surface immunoglobulin; (cIG) cytoplasmic immunoglobulin.
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