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VOLUM OMAGIAL - Facultatea de Ştiinţe ale Naturii şi Ştiinţe Agricole

VOLUM OMAGIAL - Facultatea de Ştiinţe ale Naturii şi Ştiinţe Agricole

VOLUM OMAGIAL - Facultatea de Ştiinţe ale Naturii şi Ştiinţe Agricole

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Ovidius University Annals of Natural Sciences, Biology – Ecology Series Volume 14, 2010<br />

DATA ON THE DYNAMICS OF SOME MICROBIAL GROUPS IN SOILS<br />

WITH DIFFERENT TROPHIC STATUS IN CUMPĂNA REGION (DOBROUDJA)<br />

Elena DELCĂ<br />

Ovidius University of Constanţa, Faculty of Natural Sciences and Agricultural Sciences<br />

Mamaia Avenue, No. 124, Constanţa, 900527, Romania,<br />

Doctoral school Biology, Specialization Ecology<br />

__________________________________________________________________________________________<br />

Abstract: The aim of paper was to assess the effect of administration of organic amendments on the dynamics of<br />

the abundance of microbial groups significant in nutrient cycling in soils. Abundance of total culturable bacteria<br />

ranged from 19.93x10 6 UFC/g dry soil, to 501.79x10 6 UFC/g dry soil. When soil was supplemented with manure<br />

microbial <strong>de</strong>nsity showed a significant increase 501.79x10 6 UFC/g dry soil compared with control variant.<br />

Bacterial <strong>de</strong>nsity increased significantly as value, too, following the administration of specific biofertilizers<br />

(Biovin, Bactofil Professional; Mycos Green), up to 142.13 x106UFC/g dry soil. Inorganic fertilizers did not<br />

have a positive effect on microbial <strong>de</strong>nsity values, being more or less similar to those reported for the control.<br />

Our preliminary data show that organic amendments with complex composition have a direct effect on the<br />

abundance and diversity of soil and influence indirectly the microbial metabolism and nutrient cycling rate.<br />

Keywords: humus, microorganisms, bioactivators, fertility<br />

_________________________________________________________________________________________<br />

1. Introduction<br />

To start and propose a suitable biological soil<br />

reconstruction plan it was necessary to initiate a<br />

series of observations and experiments in a<br />

characteristic agroecosystem of Dobroudja (Cumpana<br />

commune) in or<strong>de</strong>r to assess the current biological<br />

status. Using new agricultural technology, and adding<br />

different fertilizers the experiments have the aim to<br />

improve the number and activity of soil<br />

microorganism and indirectly to enhance the rate of<br />

organic matter <strong>de</strong>composition. This would improve<br />

over time the soil structure and restore the stock of<br />

humus in the soil.<br />

2. Material and Methods<br />

The experiments have taken place on a 7.5 ha<br />

plot situated in the outsi<strong>de</strong> of Cumpana, in Constanta<br />

district. Josef wheat was cultivated on the entire area,<br />

which was divi<strong>de</strong>d in 7 variants, each variant being<br />

administered a different type of fertilizer in different<br />

quantities and periods, as follows:<br />

- Variant I - only chemical fertilizers - 100kg/ha<br />

N15P25K15 in autumn, 150kg/ha NH4NO3 at the<br />

beginning of spring;<br />

- Variant II – Biovin organic fertilizer 400kg/ha and<br />

Biovin 30 of l/ha, ½ at herbici<strong>de</strong> stage and ½ at flour<br />

stage;<br />

- Variant III – gar<strong>de</strong>n soil - 15t/ha in autumn;<br />

- Variant VI – l/ha of Biovin 30, ½ at herbici<strong>de</strong> stage<br />

and ½ at flour stage;<br />

- Variant V – Biovin 150kg/ha administered during<br />

sowing, 150kg/ha NH4NO3, 40kg/ha at the beginning<br />

of spring, 50kg/ha at herbici<strong>de</strong> stage and 60kg/ha at<br />

flour stage;<br />

- Variant VI – Biovin 375kg/ha, liquid Biovin 30 of<br />

l/ha, ½ at herbici<strong>de</strong> stage and ½ at flour stage, 1mc<br />

Green Mycos, 1l Bactofil Professional;<br />

- Variant – March – were not applied amendments.<br />

Biovin Fertilizers are being administered for the<br />

first time in Dobrogea.<br />

Biovin is being produced through a<br />

technological process from grape kernels. 12 years of<br />

western research proved the following: it aerates the<br />

soil, improves it (it contains up to 70% humus<br />

makers), and purveys all plants with nutritive<br />

elements and biostimulators, it enriches the soil with<br />

ISSN-1453-1267 © 2010 Ovidius University Press

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