VOLUM OMAGIAL - Facultatea de Ştiinţe ale Naturii şi Ştiinţe Agricole

Alina Păunescu et al. / Ovidius University Annals, Biology-Ecology Series 14: 79-82 (2010)
decrease of 53.07% was found in the treated frog
individuals. Similar results were obtained at 22-24ºC
when the numbers of leukocytes decrease by 68.81%
of the control value. Similar effects have been carried
out by [10] studying the effects of chloropyrifos on
mice.
number of leukocytes/ml blood
700
600
500
400
300
200
100
0
423.9444
516.3333
226.3889 209.9444
303
195.8889
198.9444
161
control Carbetox Actara 25WG Reldan 40EC
4-6ºC
22-24ºC
Fig. 2. The influence of some insecticide upon
number of leukocytes in Rana (Pelophylax) ridibunda
The glycemia level was found to be significantly
influenced by Carbetox insecticide. Thus, as shown in
Figure 3, at a concentration of 0.01ml/g body weight,
this index increases after three weeks of treatment to
61.29% of the control value at 4-6ºC. The same
concentration of this toxic determinate, at 22-24ºC an
increase of blood glucose concentration with
212.09%. It has been reported in several studies that
hyperglycemia is one of the side effects in poisoning
by OP in subchronic exposure and in acute treatment
[11, 12, and 13]. Several studies have demonstrated
some evidence for damage in pancreatic exocrine
function after anticholinesterase
insecticide intoxication [14, 15, 16, and 17]. The
stimulation of pancreatic secretion secondary to
cholinergic stimulation seems to be responsible for
the development of pancreatitis [18, 19, 20, and 21].
The influence of Actara 25WG is also felt in the
glucose level, whose values are shown in Figure 3. Its
analysis shows an increase of glucose by 85.07%
compared to witness for animals kept at a temperature
of 4-6°C and treated with a concentration of 0.4mg/g
Actara 25WG and 153.05% for the animals kept at
22-24°C and treated with the same concentration of
toxic. Reldan 40EC in a concentration of 0.01ml/g
body weight determinate, after three weeks of
treatment, an increase of glycemia level with
127.41% as compared to the witness value in the case
81
of animals kept at 4-6ºC and with 173.59% in the
case of animals kept at 22-24ºC.
These changes occur due to inhibition of
glucose tissue by the toxic, and inhibition of Krebs
cycle and glicolise enzymes, this leading to
accumulation of glucose in the blood.
mg glucosis/ml blood
90
80
70
60
50
40
30
20
10
0
11.16667
22.72222
18
34.83333
20.66667
57.5
25.38889
62.16667
control Carbetox Actara 25WG Reldan 40EC
Fig. 3. The influence of some insecticide upon
glycemia in Rana (Pelophylax) ridibunda
4. Conclusions
4-6ºC
22-24ºC
Analyzing comparatively the influence of three
insecticides (Carbetox, Actara 25WG and Reldan
40EC) upon some physiological indices in Rana
(Pelophylax) ridibunda, we found that these decrease
(in percentage) the number of erythrocytes and
leukocytes and increase the glycemia values. Only
Reldan 40EC insecticide causes an increase in RBC.
On the other hand, the toxic effect of these
insecticides was proven to be more powerful at 22-
24ºC than 4-6ºC.
5. References
[1] BERRILL M, BERTRAM S, PAULI B, 1997 -
Effects of pesticides on amphibian embryos and
larvae. In: Green DM (ed) Amphibians in decline:
Canadian studies of a global problem. Reports
from the declining amphibian population task
force. Herpetol Conserv, 1:233–245.
[2] GREULICH K, HOQUE E, PFLUGMACHER S,
2002 - Uptake, metabolism, and effects on
detoxication enzymes of isoproturon in spawn and
tadpoles of amphibians. Environ Toxicol Saf, 52:
256–266.