VOLUM OMAGIAL - Facultatea de Ştiinţe ale Naturii şi Ştiinţe Agricole

Changes of some physiological parameters… / Ovidius University Annals, Biology-Ecology Series 14: 83-88 (2010)
water) on some physiological parameters (oxygen
consumption, breathing frequency, number of
erythrocytes 0.078125 x 10 -3 and 1.5625 x 10 -3 ml
Bravo/l water, 0.003 mg Champion/l water and 1 ml
Tiradin/l water) of the prussian carp (Carassius
auratus gibelio Bloch).
The acute and subacute toxicity of this
fungicide was evaluated in glass aquaria under semistatic
conditions.
2. Material and Methods
Determinations were made between January
2004 and October 2009 on prussian carp samples
(Carassius auratus gibelio Bloch), captured from the
surrounding rivers of Piteşti. Animals were
acclimatized for 10 days before the completion of
experiments in aquariums with a capacity of 100 l
and 50 l [10], under conditions of natural
photoperiodism, a period in which they were fed once
a day (ad libitum), at around 10 am.
After acclimatization in the laboratory, the fish
were separated in two experimental variants (lots of
10-20 fish - average weight 18 g) subjected to
fungicides.
I. Determinations of oxygen consumption and
frequency of respiratory movements at intervals of
24, 48, 72, 96, 168 and 336 hours on all samples of
these lots (depending on survival) on prussian carp
subjected to:
- I.1. Bravo 500 SC in concentrations of
0.00078125, 0.0015625, 0.003125, 0.0625, 0.0125
ml /l water and the control lot
- I.2. Champion 50 WP in concentrations of
0.003, 0.03, 0.3 and 3 mg/l water and the control lot
Tilt 250 in concentrations of 0.25, 0.5, 1, 2, 4 ml /l
water and the control lot
- I.3. Tiradin 70 PUS in concentrations of 0.01,
0.02, 0.04, 0.08, 0.16 ml /l water and the control lot
II. Hematological determinations (after one,
respectively two weeks of exposure to the fungicide,
the fishs were sacrificed to achieve intakes of blood
necessary to hematological calculations (number of
erythrocytes).
84
II.1 – fish subjected to Bravo 500 SC in
concentrations of 0.00078125, 0.0015625, ml /l
water and the control lot
II.2 – fish subjected to Champion 50 WP in
concentrations of 0.003 mg/l water and the control lot
II.3 - fish subjected to Tilt 250 in concentration
1ml /l water and the control lot
The fungicides concentrations were determined
by preliminary tests of survival. The introduction of
fish in solutions was done after their mixing and
aeration for 5 minutes. The water temperature was
16-18°C, the "immersion" solution was changed
every 24 hours, and aeration of water was continuous;
the fish were not fed during experiments to avoid
further intervention of this factor [10]. The testing
method was systematic with refreshing solution at 24
hours after the calculations of the day, in aquariums
of 100 l (50 l, respectively) for each experimental lot.
Determination of oxygen consumption was
done by means of the oximetre and Winkler method
and erythrocytes were counted with Thoma chamber,
using a small amount of blood from the caudal artery
on the optic microscope [10], [11]. The statistical
interpretation of the results was performed with
ANOVA (LSD) test.
3. Results and Discussions
The first four figures (fig.1-4) shows the
average frequency of the respiratory movements of
prussian carps exposed to the action of some
fungicide (Bravo, Champion, Tilt and Tiradin).