VOLUM OMAGIAL - Facultatea de Ştiinţe ale Naturii şi Ştiinţe Agricole

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Mariana Lupoae et al. / Ovidius University Annals, Biology-Ecology Series 14: 67-71 (2010) We found only an foliar dimorphism in the first fenophase in March when the report l/L is high 32÷37% opposite the values from May l/L=16÷28%. The mass of the leaves is 24,49 g/10 the values of the leaves is lower comparative with the population of the wild garlic from other zones (Botosani) and the mature bulbs grow until 50mm length with a mass about 2,4g. The harvested vegetal products-bulbs,flowershave a specific smell of garlic. The fitobiological and biological analysis permitted us an identify in premiere, of the subspecies studied from the Macin Mountains (Luncavita Forest) and that would be: Allium ursinum L. ssp. ucrainicum Kleopow et Oxner. The investigation of the natural population is necesarry because of the biosintetical potential therefore it can be influented by de pedoclimatic conditions from the area of the sampling of the plants. The studies undertaken by us can offer the premise of the harvest,conservation and processing of some vegetal products from wild garlic in order to improve the farmocognostic researches. 5. References [1] CIOCÂRLAN V., 2000. Flora ilustrată a României–Pteridophyta et Spermatophyta, Editura Ceres, Bucureşti, pg. 919-925. [2] SĂVULESCU T., Flora Republicii Socialiste România, Editura Academiei Republicii Socialiste România , 1966, Vol. XI, pg.193-266. [3] TITA I., 2005. Botanica farmaceutica editia a IIa, Editura Didactica si Pedagogica Bucuresti, pg. 854-863. [4 ] DJURDJEVIC L.,, Dinic A., Pavlovic P., Mitrovic M., Karadzic B., Tesevic V., 2003. Allelopathic potential of Allium ursinum L., Biochemical Systematics and Ecology 32, pg.533- 544. [5] ONCEANU (LUPOAE) Mariana, Miron Tudor Lucian, Dinica Rodica. Studiul unor principii active din specia Alium ursinum recoltată din flora spontană, publicat în rezumat, Conferinţa Naţională a Societăţii Ecologice din România, Galaţi, octombrie 2009. 71 [6] STAJNER D., POPOVIC B.M., Canadanovic- Brunet J., Stajner M., 2008. Antioxidant and scavenger activities of Allium ursinum, Fitoterapia 79, p. 303-305. [7] ARHANA SENGUPTA et al., 2004. Allium Vegetables in Cancer Prevention: An Overview, Asian Pacific Journal of Cancer Prevention, Vol 5: 237-245. [8] MIHĂILESCU R., Mitroi G. Iacob E. Miron A., Stănescu U., Gille E. , Creţu R., Ionescu E. Giurescu C. , 2008. Obtaining of phytoproducts for the cardiovascular diseases profilaxy, Note 1 Some investigation of the Allium ursinum chemical composition , The 5’th Conference on Medicinal and Aromatic Plants of Southeast European Countres , BRNO. [9] CONSTANTIN D. CHIRITA et al., 1964. Fundamentele naturalistice si metodologice ale tipologiei si cartarii stationale forestiere , Editura Academiei RPR, pag.110-113 . [10] *** FARMACOPEA ROMANA, 1993. Ediţia a-X-a , Editura Medicală Bucureşti , pg. 10-63. [11] BUCUR L.,ISTUDOR V. et al., 2002. Analiza farmacognostica, Instrument de determinare a identitatii puritatii si calitatii produselor vegetale, Editura Ovidius University Press, Constanta, pg. 7-87. [12] BERCU R., BAVARU A., 2007. Biometrical and morpho-anatomical observations on Acer monspessulanum L. (Aceraceae) leaves, Contributii Botanice, XLII, Gradina Botanica “Alexandru Borza” Cluj Napoca, pg. 105-110. [13] PETRESCU M. Cercetări privind biodiversitatea unor ecosisteme forestiere din Dobrogea de Nord, Editura Nereamia, Napocae-Tulcea, pg.61-72, 2004. [14] NEGULESCU E., SAVULESCU Al., 1957. Dendrologie, Editura Agro-Silvica de Stat, Bucuresti, pg. 184-188.
Ovidius University Annals of Natural Sciences, Biology – Ecology Series Volume 14, 2010 DINITROPHENYL DERIVATIVES ACTION ON WHEAT GERMINATION Cristina Amalia DUMITRAS -HUTANU*, *„Al. I. Cuza” University of Iasi, 11 Carol I, Iasi-700506, Romania, hutanu_amalia@yahoo.com __________________________________________________________________________________________ Abstract: Several dinitrophenyl ethers such as 2,4-dinitroanisol, 2,4-dinitrophenetol, 2,4-dinitro-1- (octadecyloxy) benzene, 3-(2,4-dinitrophenoxy)propane-1,2-diol or other similar compounds have been synthesized and tested comparatively to some well-known metabolic inhibitors and stimulators within the germination experiments. As a result, the weight of the resulted plantlets was diminished by 2,4-dinitroanisol and 3-(2,4-dinitrophenoxy)propane-1,2-diol treatments (1.15 g/lot and 32.03 mg/plantlet in the case of 2,4dinitroanisol; 0.11 g/lot and 22.3 mg/plantlet in the case of 3-(2,4-dinitrophenoxy)propane-1,2-diol). Dinitrophenyl ethers inhibited seed germination, most probably by blocking oxidative phosphorylation. A novel mechanism of action of these pesticides was discussed. Consequently,the toxicity processes of these pesticidelike compounds and metabolic inhibitors was discussed in direct relationship with their infrared absorbance and fluorescence quenching. Keywords: pesticide toxicity, dinitrophenyl ethers, dintirophenols, wheat germination. __________________________________________________________________________________________ 1. Introduction Dinitroderivatives, especially the aromatics, are frequently used as intermediates in the manufacture of pharmaceuticals, dyes, pesticides and explosives. They have multiple biological actions, being used as insecticides, fungicides, herbicides and acaricides [1, 2]. However, Environment Protection Agency in SUA (EPA) included the dinitrophenols on the list of national priorities and in concentration of 3-46 mg dinitrophenol/kg body kill; no antidote is known (max. admissible dose 70 ppb in water, EPA, 2004). It is assumed that dinitrophenols hinder the proton translocation through the mitochondrial inner membrane and therefore oxidative phosphorylation is inhibited (ATP is no longer formed and the cells deprive of essential energy supply). It is also possible that the dinitrophenols act toxically due to the inhibition of formation of some triplet states (instable biradicals) by a resonance process with the triplet structures in the living cells (A. Szent-Gyorgyi-Nobel Prize, 1957) [3, 4, 5, 6, 7, 8]. Because the existing data are inconclusive and do not support a precise action mechanism of dintrophenyl derivatives on living organisms, it was necessary to synthesize some dinitrophenols and dinitrophenyl ethers whose biological activity should be tested. The purpose of this paper is to compare the biological activity of some synthetic compounds containing the di- and nitrophenyl moiety with that of some well-known metabolic inhibitors and stimulators. Because germination experiments are easy, cheap, fast and spectacular, the testing of the action of some action of some known and newly synthesized substances on living organisms will be performed using germinating cereal seeds [3-5]. The possible mechanism of toxicity of these chemicals and pesticides are discussed in the light of the biostructural theory by Eugen Macovschi as well as the chemiosmotic theory by Peter Mitchell [6, 7, 8]. 2. Material and Methods Biological material. The wheat samples (Triticum aestivum), Henika variety, were taken from the Agricultural Research Station in Suceava. The 1000 seeds weighed 37.2 g and had a residual humidity of 12%. Chemical reagents. The reagents used were of analytic purity (Merck, Sigma, Chimopar) and the solution and the water slurries were prepared using redistilled water. Thus, ISSN-1453-1267 © 2010 Ovidius University Press
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VOLUM OMAGIAL - Facultatea de Ştiinţe ale Naturii şi Ştiinţe Agricole

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