here - Australian College of Veterinary Scientists

TABLE 2
Additional allergens used in a limited number number of horses,
obtained mainly from Stallervet
Allergen Concentration Allergen Concentration
Euroglyphus maynei 1/1.000 w/v Grass pollens
Dog dander * 1,000 PNU/ml Sweet vernal grass 10 IRU/ml
Tree pollens Yorkshire fog 1/1,000 w/v
Horse chestnut 1/1,000 w/v Perennial rye grass 10 IRU/ml
Elm 1/1,000 w/v Couch grass 1/1,000 w/v
Elder 1/1,000 w/v Weed/flower pollens
Privet 1/1,000 w/v Ragweed 10 IRU/ml
Lime 1/1,000 w/v Golden rod 1/1,000 w/v
Moulds Wall pellitory 1/1,000 w/v
Micropolysporum faeni + 100 µg/ml
Key: w/v = weight/volume dilution; PNU = protein nitrogen units; IRU = immunoreactive units;
• * = Greer allergen; + = Artuvetrin allergen
TABLE 3
Insect extracts used for equine intradermal skin testing, from Greer
Allergen Concentration
Mosquito (Culicidae) 1,000 PNU/ml
Blackfly (Simulidae) 1,000 PNU/ml
Horsefly (Tabanidae) 1,000 PNU/ml
Culicoides spp 1/1,000 w/v
1/10,000 w/v
1/100,000 w/v
Key: PNU = protein nitrogen units; w/v = weight volume dilution
Horses were examined continuously for 30 minutes and the size of the positive and
negative control wheals measured and recorded. Any wheals equal to or greater than
the mean of the size of the control wheals were considered positive and diameters in
millimetres recorded. Horses were examined again 4-6 hours later and note made of
any positive reactions (criteria as before, using the histamine diameter at the 30minute
time point). These reactions were considered immediate positive reactions.
The owner or groom examined the test site 24-36 hours later and the presence of
delayed reactions recorded and reported.
ACVSC Proceedings Dermatology Chapter Science Week 2005 33