SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists

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TOXICOKINETICS, RECOVERY, RESIDUES AND CYTOTOXICITY OF ACTP-ESTER IN
THE GOAT
C.R. Sahu*" Biplab Bagchi
Department ofZoology, Kalyani University, Kalyani, West Bengal, India
ACTP- Ester (Triclopyr butyl) marketed by Dow Elanco, USA is a selective herbicide used widely in
agricultural field. In order to study the toxicokinetic, total recovery and cytotoxicity study of ACTP- Ester
and its two potential metabolites - triclopyr acid (M]) and 3,5,6- trichloro-2 pyridinol (M2), the compound
ACTP- Ester was administered orally 396 mglkg body wt. to Black Bengal Goats (Capra capra). The
control groups were however treated with the same amount of carboxymethyl cellulose (CMC).
Blood samples were collected before (0 hr) and after 0.25,0.50,0.75, 1,2,3,4,6,8,12,36,48,60,72,84,96,
120, 144 and 168hr post administration (Pd) and were prepared for HPLC analysis following standard
protocols. Toxicokinetic parameters of ACTP Ester were determined from computerized interactive curve
fitting programme of respective blood level time profile and data were analyzed as per the standard formula
using various disposition kinetic parameters. Gross lesions for different tissues like liver, kidney, lung,
brain etc were recorded for ACTP-Ester induced cytotoxicity in goats sacrificed on 4,5,6 and 7-day pd. An
adequate blood level of ACTP-' Ester was detected as 4.94 ± 0.43 at 0.25 hr (pd). The concentration (C B
max 25.15 ± 2.62 j.lg Iml was recorded at 6 hr followed by a slow decline and minimum (C B min 3.52 ±
0.23 j.lg Iml) was detected at 60 hr pd. Kinetic behaviour of ACTP- Ester in goats followed a "two
compartmental open model kinetics". The blood level for M] and M2 was 6.52 ± 0.89 and 2.61 ± 0.13
. j.lg/ml respectively. The lower absorption rate constant suggested slow absorption of ACTPEster from
GJ.tract. The CI H value, which is equivalent to CI B value suggested that major route of elimination of
ACTP-Ester be directed through liver. Very poor CI g value suggested that urine is the minor route of
excretion of the compound. The concentration of both metabolites appears zigzag fashion, which might be
due to variable quantity as well as rate of metabolism in every unit of time.
The recovery percentage of ACTP-Ester from faeces, G.I.tract content, urine and tissues were respectively
65.38, 63.34, 66.10 and 66.24 in goats sacrificed on 4, 5, 6 and 7-day pd. Liver and lung showed gross
malformations than other tissues due to the treatment.
Keywords: Toxicokinetic, residue cytotoxicity ACTP, goat
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