SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists

C20
INVESTIGATION OF THE ACCUMULATION OF 2,4-D1CHLOROPHENOXYACETIC ACID
(2,4-D) IN RAT KIDNEYS
Handan Aydm* : University Istanbul, Faculty of Veterinary Medicine, Department of Pharmacology and
Toxicology, 34310 Avcllar, Istanbul, Turkey
A study was conducted to assess the accumulation in the kidneys of 2,4-dichlorophenoxyacetic acid (2,4­
D), the most widely used herbicide in the world, and its metabolite 2,4-dichlorophenol (2,4-DCP). 2,4-D is
eliminated in humans and animals mainly through renal excretion. Male and female Sprague-Dawley rats
were treated with 2,4-D that was introduced in their drinking water for 30 days. Group A (control group)
was fed a normal diet, while Group B was treated with 50 ppm, and Group C 100 ppm 2,4-D. In addition,
2,4-D was given daily as an oral dose, combined with their drinking water, consisting of 25 ppm in Group
D and 50 ppm in Group E. Levels of2,4-D and its metabolite 2,4-DCP in the kidneys were measured using
an HPLC method. It was observed that, though the administered doses of 2,4-D did not produce
significant toxic effects, its metabolite in particular was present at high levels in the kidneys.
Keywords: 2,4-dichlorophenoxyacetic acid (2,4-D); 2,4-dichlorophenol (2,4-DCP); HPLC
Page 238