SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists

C18
EMBLICA OFFICINALIS (FRUIT) INHIBITION OF LIVER FIBROSIS INDUCED BY CARBON
TETRACHLORIDE AND THIOACETAMIDE
SA Tasduq, P. Kaiser, H.S. Maheshwari,R.K. Johri.
Phannacology Division, Regional Research Laboratory, Jammu and Kashmir, India.
The study was carried out to investigate the antifibrotic effects of hydro-ethanolic extract of a reputed
Indian medicinal plant Emblica officinalis fruit on liver fibrosis induced by carbon tetrachloride (CCI 4 ) and
thioacetamide in rats. Liver fibrosis was assessed by measuring levels of hydroxy proline, lipid
peroxidation (LPO) based on malondialdehyde (MDA) production, microsomal drug metabolizing enzyme
CYP450 2EI measured as aniline hydroxylation, along with the antioxidant enzyme systems as catalase
(CAT), Glutathione peroxidase (GSH-Px), Glutathione (GSH). Liver Na+ K+ ATPase and y-glutathione
transpeptidase (y-gt) were studied to assess the cell membrane disintegration together with serum enzyme
activities (AST, AL T, ALP, Bilirubin). In both models of chemically induced liver fibrosis, the levels of
hydroxyproline, LPO, CYP450 2EI, Na+ K+ ATPase, y-gt along with serum enzumes remained significantly
increased with suppression of anti-oxidant defence enzymes. Treatment with a hydro-ethanolic extract of
Emblica officinalis significantly reduced the levels of hydorxyporline, MDA, , Na + K+ ATPase, y-gt and
serum enzymes. The anti-oxidant defence enzymes were restored to normal values as well. Thus this study
finds Emblica officinalis as a useful alternative source oftreatment in chronic liver fibrosis.
Keywords: Liver fibrosis, Emblica oificinalis, rat
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