SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
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M25 <br />
,---<br />
LIQUID CHROMATOGRAPHY -ELECTROSPRA Y IONIZATION MASS SPECTROMETRY<br />
FOR THE DETERMINAnON OF SELECTED BENZODIAZEPINES<br />
O.Quintela*, A. Cruz, A. de Castro M. Concheiro and M.L6pez-Rivadulla. <br />
<strong>Forensic</strong> Toxicology Service. Institute <strong>of</strong> Legal Medicine. University <strong>of</strong> Santiago de Compostela. SPAIN <br />
A simple, rapid, and sensitive method based on the use <strong>of</strong> liquid chromatography-eJectrospray ionizationmass<br />
spectrometry (LC-ESI-MS), which allows the simultaneous determination <strong>of</strong> nine Benzodiazepines<br />
(Midazolam, Bromazepam, Tetrazepam, Alprazolam, Lorazepam, Triazolam, Diazepam, Flunitrazepam<br />
and Lormetazepam) was developed. The method was applied to plasma and saliva samples. The collection<br />
<strong>of</strong> saliva samples were done with the Salivette®device. Lorazepam-d4, Diazepam-d5, and Alprazolam-d5<br />
were used as internal standards.<br />
The separation <strong>of</strong> the nine benzodiazepines was performed by using acetonitrile/O.l% formic acid in<br />
gradient mode as a mobile phase, on a XTerra RP18 5 J.lm (l50x 2.1mm I.D.) reverse phase column. The<br />
liquid-liquid extraction procedure <strong>of</strong> the compounds and their respective internal standards was carried out<br />
with diethyl ether. The quantification study was done by operating in selected ion monitoring (SIM) mode,<br />
and two mlz ratios for each compound were chosen. The method was validated for both biological samples.<br />
LOD LOQ Selected<br />
Plasma Saliva Plasma Saliva mlz ratios 8<br />
Midazolam 0.5 0.1 0.2 326.1<br />
291.3<br />
Bromazepam 0.2 5 0.5 318.0<br />
290.0<br />
Tetrazepam 0.5 0.1 0.2 289.2<br />
261.3<br />
Alprazolam 0.5 0.2 0.5 309.1<br />
281.2<br />
Lorazepam 0.5 0.2 0.5 321.0<br />
303.1<br />
Triazolam 0.5 0.1 2 0.2 343.1<br />
308.1<br />
Flunitrazepam 0.5 0.1 0.2 314.2<br />
268.2<br />
Diazepam 0.5 0.1 0.2 285.3<br />
257.2<br />
Lormetazepam 0.5 0.1 0.2 335.0<br />
289.0<br />
Lorazepam-d 4<br />
Alprazolam-ds<br />
Diazepam-ds<br />
N.D. b<br />
N.D. b<br />
N.D. b<br />
N.D. b<br />
N.D.b<br />
N.D. b<br />
N.D. b<br />
N.D.b<br />
N.D.b<br />
325.1<br />
N.D. b<br />
N.D. b 290.3<br />
N.D. b 314.2<br />
"Quantifying ions are in bold characters<br />
~.D.==Not Detected<br />
In addition, a preliminary pharmacokinetic study for two <strong>of</strong> the nine Benzodiazepines (Midazolam and<br />
Lormetazepam) was carried out in order to check the possible saliva and plasma levels correlations.<br />
Keywords: Benzodiazepines, Plasma and Saliva, LC-ESI-MS<br />
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