SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists

A7
QUANTITATIVE DETERMINATION OF AMPHETAMINES AND METHYLENEDIOXY­
AMPHETAMINES IN URINE BY MONOLITHIC COLUMN AND HPLC-DAD
A Namera*", M Nishida", M Yashiki", A Nakamoto b , Y Takei", Y Shintani", M FurunoC, H Minakuchid, K
Nakanishi", K Kimura"
a Department of Legal Medicine, Graduate School of Biomedical Sciences, Hiroshima University,
Hiroshima, b Scientific Investigation Laboratory, Hiroshima Prefectural Police Headquarters, Hiroshima, C
GL Sciences Inc., Tokyo, d Kyoto Monotech, Kyoto, e Department of Material Chemistry, Graduate School
ofEngineering, Kyoto University, Kyoto, JAPAN
Aims: A monolithic silica was developed for a new material of a micro or capillary scale HPLC. The
material consists of continuous porous silica having a double-pore structure (through-pore and meso-pore).
In this study, we developed a simple extraction of amphetamines and methylenedioxyamphetamines from a
small volume of urine by using a capillary column packed with a Cis-bonded monolithic silica.
Methods: The mixture of sample urine (0.1 ml), buffer and an internal standard was introduced into the
monolithic capillary column using a gas tight syringe. Analytes were adsorbed on. the column and
endogenous interferences were washed with an acidified buffer. Analytes were then eluted with 20-micro-L
of a mobile phase and direct injected into a HPLC.
Results: The recoveries of amphetamines and methylenedioxyamphetamines from urine were over 90%.
The calibration curves showed linearity in the range of 100 - 50,000 ng/g in urine. The coefficients of
variation of intraday and interday were below 10%.
Conclusion: These results demonstrated that the C1s-bonded column was useful for the extraction of
amphetamines in urine. This column has a potential as a new tool for the extraction of chemicals in
biological materials.
Keywords: Amphetamines, Methylenedioxyamphetamines, Monoliths
Page 121