SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
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PS <br />
POSTMORTEM DETERMINATION OF SILDENAFIL IN BLOOD<br />
Hwakyung Choi, SangHee Woo, Juseon Lee, Yooshin Park, Myungyun Pyo, Heesun Chung*<br />
National Institute <strong>of</strong> Scientific Investigation, Seoul, Korea<br />
School <strong>of</strong>Pharmacy, Sookmyung Women's. University, Seoul, Korea<br />
Sildenafil is sold as a remedy for an oral therapy for erectile dysfunction by the name <strong>of</strong> ViagraTM. It is an<br />
. inhibitor <strong>of</strong> cGMP phosphodiesterase and has a potential for cardiac risk during sexual activity in patients<br />
with preexisting cardiovascular disease. Therefore, patients who have cardiovascular problems should not<br />
use sildenafil citrate.<br />
There have been 10 sildenafil related deaths in Korea. All were males in their late thirties to sixties. To<br />
investigate the cause <strong>of</strong> death, the gastric contents and blood underwent toxicological screening. To Iml<br />
blood 50[J <strong>of</strong> 100/0 tadanafil (lnt.std) were added. Blood samples were purified by solid phase<br />
extraction using a Bond Elut-Certify .column (Varian®). Blood was loaded into a cartridge that was prewashed/conditioned<br />
with methanol and O.IMphosphate buffer. The elution was performed with ethyl<br />
acetate:ammonia (98:2). After the solid phase extraction <strong>of</strong> blood, sildenafil was analyzed with high<br />
performance liquid chromatography with photodiode array detector (HPLCIPDA). The mobile phase<br />
consisted <strong>of</strong> 0.05M triethylamine-phosphate (pH 3.0), methanol and acetonitrile (580:250: 170,v/v).<br />
Postmortem bloods were analyzed at a flow rate <strong>of</strong> 1.0mllmin with a detection wavelength <strong>of</strong> 290nm.<br />
Standard curve <strong>of</strong> spiked sildenafil into blank blood was linear with a correlation coefficient <strong>of</strong> 0.998. The<br />
limit <strong>of</strong> detection and the limit <strong>of</strong> quantification for sildenafil were 0.02 llg/mL and O.IIlg/mL, respectively.<br />
The relative recovery was 99.4%.<br />
The concentration range <strong>of</strong> sildenafil <strong>of</strong> postmortem blood was 0.00-0.70 Ilg/mL.(mean 0.166±0.067) in all<br />
ten cases. In case 6, the blood concentration <strong>of</strong> sildenafil was below the limit <strong>of</strong> quantification (0.1 Ilg/mL),<br />
however, sildenafil was confirmed in the gastric contents. In case I, 2 and 5, the victims had a medical<br />
history <strong>of</strong> cardiac disease.<br />
Key words: Sildenafil; postmortem blood concentration, HPLC/PDA<br />
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