SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
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A23 <br />
THE EXTRACTION AND ANALYSIS OF FLUNITRAZEPAM FROM WHOLKBLOODIURINE<br />
USING A NOVEL SOLID PHASE EXTRACTION PROCEDURE AND HIGH PERFORMANCE<br />
LIQUID CHROMATOGRAPHY-PHOTODIODE ARRA Y DETECTION<br />
Jeffery Hackett*, <strong>Forensic</strong> Toxicology Laboratory, Center for <strong>Forensic</strong> Sciences, Syracuse NY <br />
Jeanna M. Marraffa" Fellow in Clinical Toxicology, Poison Control Center, Syracuse NY <br />
Flunitrazepam, has been well recognized as a drug <strong>of</strong> concern within the forensic toxicology community <br />
for many years. Several methods have been published for the analysis and detection <strong>of</strong> the drug <br />
previously.I-3 The method described in this poster takes a novel yet simplified approach to the extraction! <br />
detection <strong>of</strong> flunitrazepam in bi<strong>of</strong>luids. Solid phase extraction followed by high performance liquid <br />
chromatography is employed without the need for a mass spectrometry unit for detection <strong>of</strong> low levels <strong>of</strong> <br />
the drug. Data is presented to exhibit the efficiency <strong>of</strong> the method in terms <strong>of</strong> limit <strong>of</strong> detection and <br />
quantitation_ <br />
Blood and urine were spiked with both the drug (flunitrazepam) and an internal standard (Prazepam) over a <br />
range <strong>of</strong> concentrations (O-IOng mL- 1 ). The samples were buffered with a small volume <strong>of</strong>O.IM acetic acid <br />
and applied to commercially available mixed mode (Cs/SCX) columns that had been previously <br />
conditioned. The columns were washed, dried and eluted twice, once using ethyl acetate (3 mL) and again <br />
using an elution solvent containing ethyl acetate/ acetonitrile! ammonia (3 mL). Both portions <strong>of</strong>the eluates <br />
were collected and combined. Evaporation was performed at room temperature. The residue was dissolved <br />
in 100 JlI <strong>of</strong>0.1 % aqueous trifluoroacetic acid. <br />
LC Conditions: <br />
Column:<br />
Mobile phase:<br />
Flow Rate:<br />
Column Temperature:<br />
Detection:<br />
Injection Volume:<br />
CIS, ISO x 2.lmm, 3.SJlm <br />
0.1 % Trifluoroacetic acidl Acetonitrile (67: 33) <br />
0.3 mL min-I <br />
. 40°C <br />
220, 2S0 nm <br />
SOJlL <br />
References: <br />
1. LeBeau M.A., Montgomery M_A., Wagner J.R., and Miller M.L., J <strong>Forensic</strong> Sci. 2000 <br />
Sep;4S(S): 1133-41. <br />
2. Ahrens B., Schwandt H.J., and Schutz H., Ar:neimitteiforschung. 2000 Dec;SO(12):10S7-62.<br />
3. Elian A.A., <strong>Forensic</strong> Sci Int. 1999 Apr 26;101(2):107-11.<br />
Keywords: Flunitrazepam, Solid-phase extraction, Blood and urine<br />
Page 137
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