SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists

F27
LINEAR RELATIONSHIPS OF 6.-9-TETRAHYDROCANNABINOL METABOLITES IN URINE
Scott Larson· and James D. Hutchison, Jr.
Montana State Crime Laboratory, University of Montana, Montana, USA
Many studies have focused on the metabolic patterns of marijuana and the relationship these compounds
may have to possible time of cannabis exposure, based on their concentrations in urine samples. The
objective of this study was to investigate the linear relationships of a group of 6,9-tetrahydrocannabinol
metabolites in random urine samples (n=95). In this study, gas chromatography-mass spectrometry (using
S1M profiles) was used to monitor and quantify I1-nor-6. 9 -carboxy-9-tetrahydrocannabinol (THCCOOH),
Il-Hydroxy-6. 9 -tetrahydrocannabinol (ll-OH-THC), 6,9-tetrahydrocannabinol (THC), cannabinol (CBN),
and cannabidiol (CBD). Deuterated THCCOOH-d J and 1 I-OH-THC-d J were used as internal controls. The
random urine samples were collected and submitted to the Montana State Crime Lab from adult probation
& parole, Pre-release centers, and Youth Court cases. Since these are random urine samples, there was no
way to definitely determine time of marijuana use. The results of this study indicate significant linear
relationships between some of the metabolites of THC, and also the ability to detect CBN and CBD in
random urine samples. The data presented will provide a collection ofTHCCOOH concentrations in urine
with respective comparisons to the other metabolites studied.
Keywords: 6.-9-Tetrahydrocannabinol metabolites, Urine, GCIMS
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