SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists
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A76 <br />
LC-MSIMS IDENTIFICATION OF ETHYL SULFATE - ANOTHER DIRECT MARKER FOR<br />
RECENT ETHANOL INTAKE<br />
Sebastian Dresen*·I, Wolfgang Weinmann\ Friedrich M. Wurse <br />
IInstitute <strong>of</strong> <strong>Forensic</strong> Medicine, University Hospital Freiburg, Alberstr. 9, D 79104 Freiburg, Germany, <br />
Email: Weinmann@ukl.lIni-freibllrg.de; <br />
2Psychiatric University Hospital, Wilhelm Klein Str. 27, CH 4025 Basel, Switzerland, Email: <br />
friedrich. wurst@pukbasel.ch <br />
Besides ethyl glucuronide (EtG), fatty acid ethyl esters (FAEE) and others, one more direct ethanol<br />
metabolite - ethyl sulfate (EtS) - has been detected by LC-MSIMS in human urine samples. The structural <br />
characterization has been performed by LC-MS/MS using electrospray ionization with precursor-ion scan <br />
and product-ion scan techniques. After the synthesis <strong>of</strong> a deuterated analogue, the urinary excretion pr<strong>of</strong>ile <br />
has been investigated in samples obtained from volunteers up to 44 hours after drinking known amounts <strong>of</strong> <br />
alcohol ( 9 and 18 g, respectively). Ethyl sulfate was detectable up to 24 hours in urine, Le., 16 to 22 hours <br />
longer than ethanol could be detected with a headspace GCIFID method in urine. In summary, the urinary <br />
excretion pr<strong>of</strong>ile <strong>of</strong> EtSwas similar to that <strong>of</strong> ethyl glucuronide, which is characterized by longer <br />
detectability than ethanol in urine samples and therefore is a marker for ethanol consumption covering the <br />
time range <strong>of</strong> up to approximately three days after severe ethanol abuse. The results <strong>of</strong> structural <br />
characterization, way <strong>of</strong> synthesis <strong>of</strong> the deuterated analogue, method validation data including analyte <br />
stability in urine and stock solution are presented. EtS can be used for pro<strong>of</strong> <strong>of</strong> ethanol intake besides EtG. <br />
The presented method fulfills· forensic-toxicological guidelines for compound identification by <br />
chromatographic separation, use <strong>of</strong> deuterated internal standard, quantitation and identification with <br />
precursor and two product ions being monitored as target and qualifier ions. <br />
Key words: Ethyl sulfate, Direct ethanol marker, LC-MSIMS <br />
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