SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists

M50
DETECTION OF DRUGS OF ABUSE USING ORAL FLUID WITHIN A PAROLE SETTING
Ann Snyder'*, Tiffany Miller\ Eileen Sevem 2 , Chris CiottiZ, Dean Fritch 2 , Sal Salamone 2 , Keith Kardos 2 ,
R. Sam Niedbala 2 :: 'Cuyahoga County Court of Common Pleas Laboratory, 1276 West Third St,
Cleveland, OH 44113; 2 OraSure Technologies, 150 Webster St, Bethlehem PA 18015.
A study was conducted using rapid on-site and lab based oral fluid tests as well as urine for drugs of abuse
detection in a probation facility that requires routine testing of participants. Individuals in this facility are
required to routinely visit with a probation officer. As part of their visit a urine specimen is collected, any
presence of drug is punishable and grounds for revocation of probation. This study was organized to
compare new technologies for on-site and lab-based oral fluid tests comparing them to routine urinalysis.
On-site oral fluids were determined using the UPlink Test System. UPlink is based on instrumented
analysis of lateral flow chromatography using Up-Converting Phosphors. Lab-based oral fluids were
determined using the Intercept System. The Intercept oral fluid collection device gathers an average of
OAmL of oral fluids, which is express-sent to a laboratory. After isolation of the sample, Intercept
specimens are screened using microplate assays followed by GCMSMS confirmation of positive samples.
Urine analysis was performed using Microgenics Inc. reagents on a Hitachi 917. A total of217 specimens
were tested in oral fluids. A total of 207 specimens were tested with urine due to problems with subjects
unable to provide a specimen. Positive samples were found in all fluids for all major classes of drugs
including cocaine, THC, opiates, PCP and amphetamines. True Positives and True Negatives were judged
based upon manufacturers recommendations for oral fluids and Substance Abuse and Mental Health
Services Administration Federal workplace drug testing guidelines for urine, with the exception of opiates,
which use a 300 nglmL cutoff. Overall, the % agreement when comparing on-site UPlink and lab-based
Intercept results to GCMSMS was 98.7% and 99.6% respectively. Overall % agreement between urine
screening results and GCMS was 99.8%. These results demonstrated the analytical performance of either
oral fluid test system was comparable to lab-based urine testing. However, collection of oral fluid negated
the requirements for special facilities allowing probation officers to directly observe collections.
Keywords: Oral Fluid, Urine Drug Testing, Drugs ofAbuse
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