SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists

AS1
SIMULTANEOUS ANALYSIS OF HIPPURIC ACID AND METHYLHIPPURIC ACIDS IN
URINE FROM PAINT THINNER ABUSERS USING HIGH-PERFORMANCE LIQUID
CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY
Masatoshi Morinaga*, Forensic Science Laboratory Fukuoka Prefectural Police Headquarters, 7-7 Higashi­
Koen, Hakata-ku, Fukuoka-ken 812-8576, Japan
A high-perfonnance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) assay
for the simultaneous analysis of hippuric acid and methylhippuric acids in urine is described. Urine samples
were collected from abusers sniffing paint thinner including toluene and a small amount of xylenes. These
samples were centrifuged at 3000 rpm for 10min, then diluted by distilled water 100 times and a 10ui
aliquot of sample was injected into LCIMS. 1% acetic acid, methanol, THF (90:5:5, VN) was used as a
mobile phase. The method described allows separation of hippuric acid and methylhippuric acid in less
than 40 minutes using a stainless steel column packed with octadecyl dimethylsilyiI silica. The separation
was possible for m-hippuric acid and p-methylhippuric acid. The detection limits of hippuric acid and
methylhippuric acids were 0.5ug/ml (SIR mode), respectively. The calibration curves were linear in range
of 1 to 1000ug/ml. Methylhippuric acids were detected from 36 cases of paint thinner abusers and were not
detected in urines of 10 nonnal subjects.
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Key words: Methylhippuric acids, LCIESI-MS, Paint thinner abuser
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