SOFT 2004 Meeting Abstracts - Society of Forensic Toxicologists

M33
ENHANCED SENSITIVITY FOR DRUGS OF ABUSE IN ORAL FLUID USING THE
INNOVATIVE NEW ORALSTAT TEST SYSTEM FOR ON-SITE TESTING.
Yli Remo Vallejo*, Martin Gould, Matthew Lomonico, and John Donovan: American Bio Medica
Corporation, 603 Heron Drive, Unit #3, Logan Township, New Jersey 08085
The New OralStat is an innovative test system for the rapid on-site detection ofdrugs of abuse in oral fluid
that dramatically improves the limits of detection. This test system consists of an untreated collector
sponge with a built-in sample adequacy feature, an integrated test device containing separate receptacles for
a sample buffer, gold conjugates, and immunochromatographic test strips. The device allows the lateral
flow process to be separated into discrete stages by enabling the dilution of the oral fluid sample with
buffer, the preincubation of the sample with the antibody-coated gold reagents, then allowing the sample
mixture to chromatograph on the lateral flow test strips. The use of a buffer minimizes the problems
associated with viscous saliva. The. preincubation of the sample with the antibody-coated gold reagent
greatly improves sensitivity. The device can accommodate any combination of6 drug assays.
The folfowing performance was observed in a study involving spiked oral fluid from 3 non-users:
Drug
Sensitivity (nglmU
THC (parent compound) 25
PCP 5
Amphetamine 25
Methamphetamine 25
Opiates 20
Cocaine (Benzoy I Ecgonine) 12
Benzodiazapines 25
Methadone 25
The NewOralStat test system can detect PCP, Opiates, Amphetamine, Methamphetamine, and Cocaine
metabolite at lower levels than the SAMHSA proposed cutoffs ..THC detectability at 25 ng/mL, though
still above the SAMHSA cutoff of 4 ng/mL, is a major improvement over current POCTs, improving the
detection of recent marijuana use by many hours. The results of a current study with samples from drug
users will be presented.
The New OralStat test system is visually read and requires no instrumentation to attain these levels of
sensitivity. It is however expected that the use of a reflectance reader along with agitation during the
preincubation period will further improve these limits of detection. Initial results show that by applying
such measures the THC cutoff of 4 ng/mL can be achieved.
Keywords: Oral Fluid, Drugs ofAbuse, On-Site Testing
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