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Space Grant Consortium - University of Wisconsin - Green Bay

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pyrogenic carbon. Perhaps instead <strong>of</strong> annual insecticide applications it could be stretched<br />

to 3 years, perhaps 5 years or more when applied to Cpyr.”<br />

In Cpyr-DE Slurry Experiment, EAB Mitigation, we attempted to make a long lasting non-toxic<br />

mechanical insecticide by combining Diatomaceous Earth (DE) and Cpyr. 10ml <strong>of</strong><br />

Cpyr and 10ml <strong>of</strong> DE were mixed together in 200ml <strong>of</strong> water. DE and Cpyr in water did not bind<br />

at STP when stirred. Also the slurry stuck to the glass rod when pulled from the slurry. Then, the<br />

slurry was occasionally stirred with a glass rod while heating to 464°F (240°C). At this point the<br />

glass rod was coming out <strong>of</strong> the slurry relatively clean. A drop <strong>of</strong> the heated slurry was placed on<br />

a microscope slide. The following was noted in an email 28<br />

to the team, “I put a sample on a<br />

slide and the DE in some places was absorbing the carbon into the DE cells and in some<br />

places tiny specs <strong>of</strong> carbon were adhering to the DE. It seems that if I "cooked" it long<br />

enough that the DE would assimilate the carbon. In any case I think this is a significant<br />

find... that I can get Cpyr and DE to bind. It might turn out to be a valuable insecticide.”<br />

Applications: Pyro-DE injected under the bark where EAB and Mountain Pine Beetle<br />

infestations are suspected could mitigate that infestation. Furthermore, the value <strong>of</strong> Cpyr cannot<br />

be underestimated for increasing plant fertility and reducing plant stress, especially in infested<br />

trees. Infested, dying and decaying trees are now considered a massive source <strong>of</strong> CO2 emission<br />

rather than them being a natural carbon sink. We propose that research along these lines is<br />

applicable to forestry habitat and agricultural sustainability.<br />

MAX-X. Below is a list <strong>of</strong> future experiments but, to place Star on the moon by 2016, we will<br />

need to conduct all-encompassing or maximum experiments (MAX-X) over the next couple <strong>of</strong><br />

years. So, what would a MAX-X experiment look like? During the lunar day Star would be<br />

exposed to high-heat and radiation while growing in a vacuum. During the lunar night Star<br />

would be exposed to cryogenic temperatures and radiation while growing in a vacuum. MAX-X<br />

acclimation experiments would give Star the greatest chance to survive on the moon.<br />

Future Experiments.<br />

1. To increase the germination rate <strong>of</strong> O. seeds.<br />

2. Study effects <strong>of</strong> lunar night/day cycling on germination rate <strong>of</strong> O. seeds.<br />

3. Do flowers develop during lunar cycling?<br />

4. After artificial pollination, how do fruits develop during lunar cycling and how much<br />

time do they need?<br />

5. Test O. survival in extreme thermal cycling under specified conditions such as humidity.<br />

6. Metrics <strong>of</strong> O. adaptability.<br />

7. Does lunar cycling increase O. adaptability to extreme conditions?<br />

8. Can O. fragilis, O. phaeacantha, and O. polyacantha tolerate the heat as well as the cold?<br />

9. Can O. fragilis, O. phaeacantha, and O. polyacantha pass the “cold tolerance factor”<br />

gene to an O. ficus-indica seedling via grafting?<br />

10. How will the O. ficus-indica seedling develop on a cold tolerant plant?<br />

11. How will and O. fragilis, O. phaeacantha or O. polyacantha seedlings grow on a O.<br />

ficus-indica graft and would they be edible?<br />

12. Can organic wastes supplement N in lunar regolith?<br />

28 Wednesday – March 11, 2009, 5:13 PM.<br />

31

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