Orion AquaMate 7000 Vis Orion AquaMate 8000 UV-Vis - Daigger

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25 Calculations for SoftwareCalculation Calculation(s) GraphsLinear regressionthrough zeroA = a 1 * (c)Where:A = absorbancec = concentrationa 1 = slopeThe slope is calculated as:a 1 = SXY/SXXThis model requires:• Slope is not equal to zero or infinity• At least one standard data point with concentration>0Segmented model• The absorbance of the 0 concentration blank = 0AThe segmented model requires:• Data for at least two standard data points withdifferent concentrations and absorbancesValidity of standardcurves• Slopes of all segments must be ascending (positive)or descending (negative)A(c 1 ) > A(c 2 ) for all c 1 > c 2orA(c 1 ) < A(c 2 ) for all c 1 > c 2Where:A = absorbancec 1 , c 2 = concentrationIf this is not the case, there will be more than onesolution within the specified domain, and the message“Curve cannot be used to determine sampleconcentrations – it may produce ambiguous results”will appear when the curve is viewed.Valid nonlinear standard curveInvalid nonlinear standard curve136 Orion AquaMate 7000 Vis and Orion AquaMate 8000 UV-Vis Thermo Scientific
25 Calculations for SoftwareCalculation Calculation(s) GraphsStatistics(Linear regressiongeneral case) y y 2i iN n 1Where:N = Degree of polynomialr SSXYSQX SQYLinear regressionthrough zero modelAbsorbance ratioAbsorbanceDifferenceThe calculation for the correlation coefficient appliesonly to first order linear regression curves (first degreepolynomials). Abs1AbsResult =AbsorSYY a1 SXYN 12Abs Abs1Abs Abs2refref1 factor 1Abs2 factor2orAbs Absfactor Abs Abs21 ref12reffactorThermo Scientific Orion AquaMate 7000 Vis and Orion AquaMate 8000 UV-Vis 137
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UV-VisOrion AquaMate 7000 VisOrion
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CContentsPreface . . . . . . . . .
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ContentsChapter 24 Parameters . . .
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PPrefaceCongratulations on your pur
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1Spectrophotometer BasicsThis chapt
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1 Spectrophotometer BasicsSpectroph
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1 Spectrophotometer BasicsSelecting
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2Setting Up the InstrumentSetting u
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2 Setting Up the InstrumentSetting
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2 Setting Up the InstrumentSetting
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3AccessoriesThis chapter briefly de
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3 AccessoriesCell Holders and Cell
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3 AccessoriesExternal PrintersNote
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4Sample Positioner SettingThe spect
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5Cell CorrectionEvery test setup sc
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5 Cell CorrectionCell Correction7.
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6Managing Stored TestsThe instrumen
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6 Managing Stored TestsSaving a Tes
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6 Managing Stored TestsLock/UnlockN
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7SmartStartThe SmartStart feature l
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8Concentration UnitsThis chapter co
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9Calculator FunctionTo use the Calc
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10Water Analysis ApplicationThe Wat
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10 Water Analysis ApplicationRunnin
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10 Water Analysis ApplicationProgra
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10 Water Analysis ApplicationUsing
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11Abs and %T Measurements—Basic A
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12Abs and %T Measurements—Advance
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12 Abs and %T Measurements—Advanc
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13Basic Concentration Measurements
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13 Basic Concentration Measurements
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14Concentration Measurements—Adva
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14 Concentration Measurements—Adv
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15ScanningThe wavelength scanning a
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15 ScanningScanning a Sample2. Plac
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15 ScanningViewing and Manipulating
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16MultiwavelengthThe Multiwavelengt
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16 MultiwavelengthTaking Measuremen
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17Absorbance RatioThe Absorbance Ra
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17 Absorbance RatioMeasuring Sample
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18Absorbance DifferenceThe Absorban
Page 95 and 96: 18 Absorbance DifferenceMeasuring S
Page 97 and 98: 193-Point NetThe 3-Point Net applic
Page 99 and 100: 19 3-Point NetTaking Measurements2.
Page 101 and 102: 20Concentration Measurements—Stan
Page 103 and 104: 20 Concentration Measurements—Sta
Page 109 and 110: 21KineticsThe Kinetics application
Page 111 and 112: 21 KineticsMeasuring SamplesParamet
Page 113: 21 KineticsRecalling and Recalculat
Page 116 and 117: 21 KineticsRescaling and Recalculat
Page 118 and 119: 22 Performance VerificationTroubles
Page 120 and 121: 22 Performance VerificationWaveleng
Page 122 and 123: 22 Performance VerificationResoluti
Page 124 and 125: 22 Performance VerificationPhotomet
Page 126 and 127: 22 Performance VerificationStray Li
Page 128 and 129: 22 Performance VerificationStray Li
Page 130 and 131: 23 MaintenanceRoutine CareCleaning
Page 132 and 133: 23 MaintenanceChanging the FuseIMPO
Page 134 and 135: 23 MaintenanceReplacing the Tungste
Page 136 and 137: 23 MaintenanceReplacing the Tungste
Page 138 and 139: 24 ParametersParameterCalculatorCel
Page 140 and 141: 24 ParametersParameterFactor 2Facto
Page 142 and 143: 24 ParametersParameterNumber of Sta
Page 144 and 145: 24 ParametersParameterDescriptionTa
Page 148: 25 Calculations for SoftwareCalcula
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