Detection and Expression of Biosynthetic Genes in Actinobacteria ...
Detection and Expression of Biosynthetic Genes in Actinobacteria ...
Detection and Expression of Biosynthetic Genes in Actinobacteria ...
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BERVANAKIS, G. Chapter 3: RESULTS161 240{S.coelicolor.SC6G9.15} EEFGVRGPVQTVSTGCTSGLDAVGYAY.HAVAEGRVDVCLAGAADSPISPITMACFDAIKATSPNNDDPAHASRPFDADR{S.halstedii} ETFGAQGPVQTVSTGCTSGLDAVGYAY.HAIAEGRADVCLAGASDSPISPITMACFDAIKATSPSNDDPEHASRPFDARR{S.curacoi} ERFGAQGPVQTVSTGCTSGLDAVGYAF.HTIEEGRADVCIAGASDSPISPITMACFDAIKATSPNNDDPEHASRPFDAHR{A1488} ...................HRAVGYGS.QLIEDGDADVVISGASDAPISPISMACFDTIRATTGNNSDPEHAAKPFDARR{A3023} .....................AVGYAF.HTISEGRADVWIAGASDSPISPITMACFDAIKATSAQNDDPDHASRPFDGRRConsensus ------GP---VS-GCTSG-DAVGYA--H-I-EGRADV-IAGASDSPISPITMACFDAIKATS--NDDP-HASRPFDA-R241 320{S.coelicolor.SC6G9.15} NGFVMGEGAAVLVLEDLEHARARGADVYCEVSGYATFGNAYHMTGLTKEGLEMARAIDTALDMAELDGSAIDYVNAHGSG{S.halstedii} NGFVMGEGGAVLVLEELEHARARGADVYCELAGYATFGNAHHMTGLTREGLEMARAIDTALDMARLDGTDIDYVNAHGSG{S.curacoi} DGFVMGEGAAVLVLEELEHARARGAHVYCEIGGYATFGNAYHMTGLTSEGLEMARAIDVALDHARVDPTDIDYVNAHGSG{A1488} DGFVMGEGAAVLVLEELEHARRRGAEVYCEISGYANRCNAFHMTGLRPDGLEMAEAIRLAMDQGRIDADEVGYVNAHGSG{A3023} DGFVMGEGAQC.......*CWRTTTTRGPXARTSSARSSATH......XXQQLPXMXLXQLRLAXXXPTHTXX.......Consensus -GFVMGEGAAVLVLEELEHAR-RGA-VYCEI-GYA---NA-HMTGL---GLEMA-AI--ALD-A--------YVNAHGSG321 400{S.coelicolor.SC6G9.15} TQQNDRHETAAVKRSLGEHAYATPMSSIKSMVGHSLGAIGSIELAACVLAMAHQVVPPTANYTTPDPECDLDYVPREARE{S.halstedii} TQQNDRHETAAVKRSLGEHAYRTPMSSIKSMVGHSLGAIGSIEVVACVLALAHQVVPPTANYETPDPECDLDYVPREARE{S.curacoi} TRQNDRHETAAVKKSLGAHAYDTPMSSIKSMVGHSLGAIGAIEVVACVLALARQVVPPTANYETPDPECDLDYVPRTARP{A1488} TNR.....TTAKKXXXX...............................................................{A3023} ................................................................................Consensus T-QND-HETAA-K--L---A---P-SSIKSMVGHSLGAI---E--A--L-----VVPPT-N----DPECDLDYV----R-Figure 23.Multiple sequence alignment <strong>of</strong> β-Ketoacyl synthase (KS α ) genes from type II PKS <strong>of</strong> spore pigment genes <strong>in</strong>act<strong>in</strong>obacteria S. coelicolor SC6G9.15 (SWISS-PROT # P23155;whiE); S. halstedii (SWISS-PROT # Q05356;sch1);S. curacoi (SWISS-PROT # Q02578;curD) with amplified environmental KS a genes from environmental act<strong>in</strong>obacteriaisolates A1488 <strong>and</strong> A3023.79
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