Detection and Expression of Biosynthetic Genes in Actinobacteria ...

BERVANAKIS, G.Chapter 3: RESULTSwhich were obtained with six of the Cerylid isolates (Figure 30). A qualitativeassessment only could be made based on the presence of the amplified band, as nodirect sequencing was performed due to the lack of time.Table 43: Comparison of nucleotide and amino acids from amplified dTDP-glucosesynthase gene from Streptomyces griseus DSM40236 Type Strain with dTDP-glucosesynthase from different actinobacterial strains. Percentage similarity obtained usingthe FASTA database search.% SimilarityActinobacteria 1 dTDP-glucoseStreptomyces griseus DSM40236synthase gene Nucleic AcidAmino AcidActinoplanes sp. acbA 60 58SE50/110M.olivasterospora^ fotD 59 56S.spinosa* Gtt 62 60S.antibioticus Tu99 oleS 73 69S.argillacens mtmD 76 69S.bluensis blmD 83 77S.collinus napG 59 54S.cyanogenus S136 lanG 72 61S.fradiae urdG 72 60S.glaucescens StrD 81 79S.griseus strD 82 78S.kanamyceticus knaD 72 80S.peucetius dnrL 70 67S.rimosus prmD 71 63S.rishiriensis couV 59 57S.spheroides novV 58 55S.violaceoruber gra-orf16 69 55S.viridochromogenes avid 71 621 All Streptomycete species are indicated by the letter S., non-Streptomycetes Actinobacteria aredepicted in the following manner * Saccharopolyspora., ^Micromonospora.(kb)2.01.51.00.70.50.40.30.20.11 2 3 4 5 6 7 8 9 10 11Figure 30: Amplification of 0.37 kb glucose dTDP glucose synthase fragment byPCR from environmental actinobacterial cultures. Lane 1: Molecular weightmarker in kilobases (kb). Lane 2: Positive control 16SrDNA 27f and 765rprimers.,Lane 3: A2010., Lane 4: A1113., Lane 5: A2707., Lane 6: A350., Lane 7:A2056., Lane 8: A371., Lane 9: A1990., Lane 10: A096., Lane 11: A2834._____________________________________________________________________91