Detection and Expression of Biosynthetic Genes in Actinobacteria ...

BERVANAKIS, G.__________________________________________________________________________________ Chapter 3: RESULTS901 990a.msf{AB024980} CTCGACCAGGCCCGGCTCGATCCCACGCGGATCGACTACGTCAAC GCACACGGCTCTGGCACCCGGCAGAACGACCGGCACGAGACCGCCa.msf{AB024981} CTCGGCCAGGCACGGCTCGACCCCACGGCGATCGACTACGTCAAC GCGCACGGCTCCGGCACCCGGCAGAACGACCGCCACGAGACGGCGa.msf{AB024975} CTCGACATCGCGCGGCTGGATCCCTCGGACGTGGACTACGTCAAC GCGCACGGCTCGGCGACCAAGCAGAACGACCTCCACGAGACGGCGa.msf{Z25538} CTCGACATCGCGCGCGTCGATCCGTCGGAGGTGGACTACGTCAAC GCCCACGGTTCGGCGACCAAGCAGAACGATCTCCATGAGACGGCGa.msf{AB024977} CTGGACCGGGCGCGGATGGACCCGACAGACATCGACTACGTCAAC GCCCATGGCTCGGCGACCAAACAGAACGACCTCCACGAGACGGCGa.msf{AB024976} CTCGACCGCAGCCGGATGAACCCCGAGGACGTCGGCTACGTCAAC GCGCACGGCTCGGCGACCAAGCAGAACGACCGGCACGAGACCGCCa.msf{AB024970} CTCGACGACGCCCGCATCGACCGCGAAGCCGTCGGTTACGTCAAC GCGCACGGCACGGCCACCCGGCAGAACGACATCCACGAGACGGCCa.msf{AB024971} CTCGACGACGCCCGCATCGACCGCGAAGCCGTCGGTTACGTCAAC GCGCACGGCACGGCCACCCGGCAGAACGACATCCACGAGACGGCCa.msf{AB024972} CTCGACGACGCCCGGATCGACCGCACCTCCGTCGGCTACGTCAAC GCCCACGGCACCGCCACCCGGCAGAACGACATCCACGAGACGGCCa.msf{AB024973} CTCGACGACGCCCGGATCGACCGCGAGGCCGTCGGCTACGTCAAC GCGCACGGCACAGCCACCCGGCAGAACGACATCCACGAGACGGCCa.msf{AB024974} CTCGACGAGGCCCGCCTCGGCCCCGACGCGCTCGGCTACGTCAAC GCCCACGGCACCGCCACCAAGCAGAACGACGTGCACGAGACCGCCa.msf{AB021222} CTGGACGAGGCCCGCCTGAACCCCGCGGACGTCCACTACGTCAAC GCCCACGGTTCGGGGACCAAGCAGAACGACCGGCACGAGACGGCCa.msf{X89899} CTCGACGAGGCACGCCTGGACCCCACGGCCGTCGACTACGTCAAC GCGCACGGCTCCGGCACCAAGCAGAACGACCGGCACGAGACCGCCa.msf{AF098965} CTCGACGAGGCCCGCCTCGACCGGACCGCCGTCGACTACGTCAAC GCGCACGGCTCCGGCACCAAGCAGAACGACCGCCACGAGACCGCCa.msf{X16300} CTCGACGAGGCCCGGCTGGACCGTACGGCGGTCGACTACGTCAAC GCGCACGGCTCCGGCACCAAGCAGAACGACCGCCACGAGACGGCCa.msf{X63449} CTCGACGAGTCCCGCACGGACGCGACGGACATCGACTACATCAAC GCGCACGGCTCCGGCACCCGGCAGAACGACCGCCACGAGACAGCGa.msf{L26338} CTCGCCGAGAGCGGGACCGACCCCGCCGCCGTGGACTACGTCAAC GCCCACGGCTCCGGCACCAAGCAGAACGACCGGCACGAGACCGCCa.msf{AB024978} CTCGACGAGGCGCGGCTCGCCCCGGAGGCCATCGACTACGTCAAC GCGCACGGCTCCGGCACCAAGCAGAACGACCGGCACGAGACGGCCa.msf{AF126429} CTCGACGAGGCGCGGATGAACCCCACCGAGATCGACTACATCAAC GCGCACGGCTCCGGCACCAAGCAGAACGACCGCCACGAGACCGCAa.msf{Z11511} CTGGACGAGGCCCGCCTCAACCCCGAGCAGGTCGACTACATCAAC GCCCACGGCTCGGGCACCAAGCAGAACGACCGGCACGAGACGGCCa.msf{L24518} CTGGACGGCGCGAAGATGGGCCCGCAGGACGTCGACTACGTCAAC GCGCACGGCTCGGGCACCAAGCAGAACGACCGGCACGAGACCGCGa.msf{X77865} CTGGACGAGGCCCGGCTCGACGCGTCGGCGGTGGACTACGTCAAC GCGCACGGTTCCGGCACCCGCCAGAACGACCGGCACGAGACGGTCa.msf{AB024979} CTGGCCCGGTCCGGGACCGCGCCGGAGGACATCGACTACATCAAC GCCCACGGCTCGGGCACCCTGCAGAACGACCGGCACGAGACGGCGa.msf{AJ224512} CTCGCCCAGGCGGGCAAGGCGCCGGCTGACGTCGACTACGTCAAC GCCCACGGTTCCGGCACCCGGCAGAATGACCGTCACGAGACGGCGa.msf{AB019690} ATGGAGGCGGCGCGGATCGGGCCGGACGACATCGACTACATCAAC GCGCACGGGTCCGGCACCCGGCAGAACGACCGGCACGAGACCGCGa.msf{X15312} CTGGACCAGGCCCGCCGCACCGGCGACGACCTGCACTACATCAAC GCGCACGGCTCCGGCACCCGGCAGAACGACCGGCACGAGACGGCGConsensus CT-GAC---GC-CG-------C-----G---T-G-CTAC-TCAAC GC-CACGG--C-G--ACC--GCAGAACGAC---CACGAGAC-GC-Primer act06r3’ GTCTCGTGGCGGTCGTTCTGC 5’Figure 21. Comparison of the nucleic acid sequences of KSα genes. The 26 sequences were compared by the PILEUP method. Respective accession numbers areshown in brackets after species name. S.coelicolor (X63449)., S.nogalater (AB024981)., S.steffisburgenesis (AB024979)., S.albus (AB024978)., S.platensis (AB024977).,S.aureofaciens (AB024976)., S.albofaciens (AB024975)., S.purpurascens (AB024974)., S.tauricus (AB024973)., S.capoamus (AB024972)., S.galilaeus (AB024971).,S.bobili (AB024970)., S.griseus (X77865)., S.glaucescens (X15312)., S.violaceoruber (X16300)., S.rimosus (Z25538)., S.argillaceus (X89899)., A.verrucosospora(AB019690)., S.venezuelae (AB024980)., S.venezuelae (AF126429)., S.nogalater (AJ224512)., S.rochei (AB021222)., S.roseofulvus (L26338)., S.cinnamonensis (Z11511).,S.arenae (AF098965)., K.aridum (L24518).____________________________________________________________________________________________________________________76