Detection and Expression of Biosynthetic Genes in Actinobacteria ...

BERVANAKIS, G.Chapter 3: RESULTSTable 40: Comparison of PCR amplified KS gene from Cerylid cultures A0350 andA1113 sequence percentage similarity with mPKS genes using the FASTA databasesearch.% SimilarityActinobacteria § PKS Gene A0350Amino NucleicA1113Amino NucleicAcid Acid Acid AcidA. mediterranei PKS 50 70 61 77M. griseorubida mycAORF1 47 68 72 75M. inyoensis a PKS 69 72 76 77M. megalomicea. megAII 49 70 64 77subsp.nigraS. erythraea*S. spinosa*S. albuseryAspnCPKS55 7151 6673 7567 8061 7474 76S. antiibioticus oleAI 48 71 58 60S. avermitilis aveA2 55 71 59 75S. bluensis PKS 70 73 59 62S. caelestis nidA1 49 69 63 77S. cinnamonensis PKS 48 70 59 77S. coelicolor SC2C4.04C 52 73 63 74S. griseus PKS 46 68 58 79S. hydroscopicus PKS 52 72 65 73S. kanamyceticus PKS 52 66 64 78S. natalensis pimSO 65 68 68 71S. noursei. nysI 53 70 60 74S. rimosus PKS 60 65 71 74S. rochei lkm 70 73 60 75S. sp. MA6548 fkbA 67 70 75 76S. venezuelae pikAII 53 71 64 79S.viridochro- aviM 65 68 66 72mogenesS. netropsis^ PKS 58 60 59 66§All Streptomycete species are indicated by the letter S., non-StreptomycetesActinobacteria are depicted in the following manner * Saccharopolyspora., ^Streptoverticillium., a MicromonosporaMultiple sequence alignments of partial amino acid sequence translated from theamplified DNA sequence from isolates A0350 and A1113, indicated that they alsocontained conserved domains from KS genes (purple coloured boxes figure 26)._____________________________________________________________________85