Detection and Expression of Biosynthetic Genes in Actinobacteria ...
Detection and Expression of Biosynthetic Genes in Actinobacteria ...
Detection and Expression of Biosynthetic Genes in Actinobacteria ...
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BERVANAKIS, G.Chapter 3: RESULTSFigure 27. Unrooted neighbour-jo<strong>in</strong><strong>in</strong>g phylogenetic tree constructed fromam<strong>in</strong>o acid sequences <strong>of</strong> KS genes fragments from type I polyketide synthases.The am<strong>in</strong>o acid sequence accession numbers for the reference stra<strong>in</strong>s are as follows;A. mediterranei (AAC01710); S. hygroscopicus (CAA60460); S. sp.MA6548(AAC68815); S. natalensis (CAB41040); S. antibioticus (AAA19695);M.sagamiensis (AAD43312); Streptoverticillium netropsis (Q9XCE8);M. griseorubida (BAA76543); S.avermitilis (BAA84474); S. erythreae (CAA39583);S. fradiae (AAB66504); S. sp<strong>in</strong>osa (AAG23264); S. caelestis (AAC46024);S. venezuelae (AAC69329) <strong>and</strong> amplified KS from the Cerylid isolates A1113 <strong>and</strong>A0350. The sequences from the reference stra<strong>in</strong>s where obta<strong>in</strong>ed from GenBank.The am<strong>in</strong>o acids encoded by 15 known macrolide PKS antibiotic gene fragments wereused as a basis for classification <strong>of</strong> the Cerylid isolates. The phylogenetic treeresolved sequences <strong>in</strong>to four major groups (Figure. 27). The A1113 sequence issimilar to Sachharopolspora erythrae eryA the PKS responsible for the production <strong>of</strong>erythromyc<strong>in</strong>, while the A0350 sequence forms a dist<strong>in</strong>ctive branch <strong>in</strong> thephylogenetic tree._____________________________________________________________________87
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