Detection and Expression of Biosynthetic Genes in Actinobacteria ...

BERVANAKIS, G.Chapter 3: RESULTSTable 52. Characterisation of ethyl acetate extracted metabolites from isolatenumber A2707 on different solid-substrate fermentation media.Media Well-Type TLC Extract ColourType* Assay^ Number ofBandsWSF29 ++ 4 Yellow tingeWBYLF42 ++ 3 YellowWBYH 2 0 ++ 3 YellowWOH 2 0 - 8 Yellow tingeWOSF29 ++ 4 OrangeRSF29 ++ 5 ClearRH 2 0 ++ 2 YellowRLF42 ++ 5 YellowRYSF29 ++ 5 YellowRYLF42 ++ 7 Pale YellowRYH 2 0 ++ 6 YellowBYH 2 0 ++ 3 YellowBYLF42 ++ 2 YellowBYSF29 ++ 3 Pale YellowPH 2 0 - ND ClearPLF42 ++ 2 ClearPSF29 - ND ClearPHSF29 ++ 3 Yellow TingePHLF42 + 4 ClearCH 2 0 ++ 4 YellowCLF42 ++ 5 YellowLSAH20 ++ 1 OrangeBH 2 0 - 7 YellowBSF29 ++ 5 YellowBLF42 ++ 6 Yellow* Fermentation media key:H 2 0 (water)Liquid Solutions: LF42 (trace elements), SF29 (minerals)Solid Substrate: W = Wheat Bran., WBY = Whole Barley., WO = WholeOats., R= Rice., RY = Rye., BY = Barley., P = Perlite., PH = Psyllium Husk.,C = Corn., LSA = Linseed, Sunflower Kernals, Almonds., B = Burghal^ Antimicrobial activity was measured against M. luteus.^ Zones of Inhibition: + 8 – 15 mm, ++ 16 – 25 mm, +++ >25 mm,- no inhibition, ND = not detectedThe EtAc extract color was also recorded to observe if any color changes occurred asa result of the extraction process. The EtAc extract of culture A2707 which has anorange appearance in liquid media produced two colorations on solid media eitheryellow or clear extracts (Table 52). The yellow EtAc extracts were the more bioactiveand expressed a greater number of metabolites than the clear extracts._____________________________________________________________________101