_____________________________________________________________________________________Jonsbu, E., McIntyre, M. <strong>and</strong> Nielsen, J. (2002). The <strong>in</strong>fluence <strong>of</strong> carbon sources <strong>and</strong>morphology on nystat<strong>in</strong> production by Streptomyces noursei. J. Biotechnol. 95:133-144.Julien, B., Shah, S., Ziermann, R., Goldman, R., Katz, L <strong>and</strong> Khosla, C. (2000). Isolation<strong>and</strong> characterization <strong>of</strong> the epothilone biosynthetic gene cluster from Sorangiumcellulosum. Gene 249:153-160.Jung, H.M., Kim, S.Y., Hyun, H.H. <strong>and</strong> Lee, J.K. (2002). Ca 2+ <strong>and</strong> Cu 2+ supplementationaugments vancomyc<strong>in</strong> production by Amycolatopsis orientalis. Biotechnol. Lett. 24:293-296.Kaiser, D., Onken, U., Sattler, I <strong>and</strong> Zeeck, A. (1994). Influence <strong>of</strong> <strong>in</strong>creased dissolvedoxygen concentration on the formation <strong>of</strong> secondary metabolites by manumyc<strong>in</strong>produc<strong>in</strong>gStreptomyces parvulus. Appl. Microbiol. Biotechnol. 41:309-312.Kakavas, S.J., Katz, L. <strong>and</strong> Stassi, D. (1997). Identification <strong>and</strong> characterization <strong>of</strong> theniddamyc<strong>in</strong> polyketide synthase genes from Streptomyces caelestis. J. Bacteriol.179(23):7515-7522.Kang, J-G <strong>and</strong> Roe, J-H. (1998). Sigma factors <strong>of</strong> RNA polymerase from S.coelicolor.Act<strong>in</strong>omycetol. 12(2):129-133.Katz, E <strong>and</strong> Weissbach, H. (1962). Biosynthesis <strong>of</strong> act<strong>in</strong>omyc<strong>in</strong> chromophore: enzymaticconversion <strong>of</strong> 4-methyl-3-hydroxyanthranilic acid to act<strong>in</strong>oc<strong>in</strong>. J. Biol. Chem. 237:882-886.Katz, L. (1997). Manipulation <strong>of</strong> modular polyketide synthases. Chem. Rev. 97:2557-2575.Katz, L <strong>and</strong> Donadio, S. (1993). Polyketide synthesis: prospects for hybrid antibiotics.Annu. Rev. Microbiol. 47:875-912.Katzer, W., Blackburn, M., Charman, K., Mart<strong>in</strong>, S., Penn, J <strong>and</strong> Wrigley, S. (2001).Scale-up <strong>of</strong> filamentous organisms from tubes <strong>and</strong> shake-flasks <strong>in</strong>to stirred vessels.Biochem. Eng. J. 7:127-134.Kealey, J.T., Liu, L., Santi, D.V., Betlach, M.C <strong>and</strong> Barr, P.J. (1998). Production <strong>of</strong> apolyketide natural product <strong>in</strong> nonpolyketide produc<strong>in</strong>g prokaryotic <strong>and</strong> eukaryotic hosts.Proc. Nat. Acad. Sci. USA. 95:505-509.Keeratipibul, S., Sugiyama, M., Nimi, O. <strong>and</strong> Nomi, R. (1984). Streptothric<strong>in</strong> productionby a new isolate <strong>of</strong> Streptomyces from Thail<strong>and</strong> soil. J. Ferment. Technol. 62(1):19-28.____________________________________________________________________________________149
_____________________________________________________________________________________Khetan, A., Malmberg, L.H., Kyung, Y.S., Sherman, D.H, <strong>and</strong> Hu, W.S. (1999).Precursor <strong>and</strong> c<strong>of</strong>actor as a check valve for cephamyc<strong>in</strong> biosynthesis <strong>in</strong> Streptomycesclavuligerus. Biotechnol. Prog. 15:1020-1027.Khosla, C. (1997). Harness<strong>in</strong>g the biosynthetic potential <strong>of</strong> modular polyketidesynthases. Chem. Rev. 97:2577-2590.Khosla, C. “Comb<strong>in</strong>atorial biosynthesis <strong>of</strong> “unnatural” natural products.” Comb<strong>in</strong>atorialchemistry <strong>and</strong> molecular diversity <strong>in</strong> drug discovery. Ed. Gordon, E.M <strong>and</strong> Kerw<strong>in</strong>,J.F.Jr. New York. USA: Wiley-Liss. Inc, 1998. 401-417.Khosla, C <strong>and</strong> Zwada, R.J.X. (1996). Generation <strong>of</strong> polyketide libraries via comb<strong>in</strong>atorialbiosynthesis. Trends Biotechnol. 14:335-341.Kim, E.S., Bibb, M.J., Butler, M.J., Hopwood, D.A <strong>and</strong> Sherman, D.H. (1994).Sequences <strong>of</strong> the oxytetracycl<strong>in</strong>e polyketide synthase-encod<strong>in</strong>g otc genes fromStreptomyces rimosus. Gene 141:141-142.Kim, C.J., Chang, Y.K., Chun, G.T., Jeong, Y.H. <strong>and</strong> Lee, S.J. (2001). Cont<strong>in</strong>uousculture <strong>of</strong> immobilized Streptomyces cells for kasugamyc<strong>in</strong> production. Biotechnology17: 453-461.K<strong>in</strong>ashi, H.(1994). L<strong>in</strong>ear plasmids from act<strong>in</strong>omycetes. Act<strong>in</strong>omycetol. 8(2):87-96.Kirschn<strong>in</strong>g, A., Beehtold, A.F-H <strong>and</strong> Rohr, J. “Chemical <strong>and</strong> biochemical aspects <strong>of</strong>deoxysugars <strong>and</strong> deoxysugar oligosaccharides.” Bioorganic chemistry. deoxysugars,polyketides <strong>and</strong> related classes: synthesis, biosynthesis, enzymes. Vol.188. Top. Curr.Chem. Ed. Rohr, J. Heidelberg: Spr<strong>in</strong>ger-Verlag, 1997. 2-84.Kook, R-J <strong>and</strong> Nam, D-H. (1997). Clon<strong>in</strong>g <strong>of</strong> Isopenicill<strong>in</strong> N synthase gene fromLysobacter lactamgenus. J. Microbiol. Biotechnol. 7(6):373-377.Kota, K.P <strong>and</strong> Sridhar, P. (1999). Solid state cultivation <strong>of</strong> Streptomyces clavuligerus forcephamyc<strong>in</strong> C production. Process Biochem. 34:587-590.Kotra, L.P., Golemi, D., Vakulenko, S <strong>and</strong> Mobashery, S. (2000). Bacteria fight back.Chem. Ind. 341-344.Krallis, M. <strong>and</strong> Kirby, R. (1998). Development <strong>of</strong> a PCR.southern dot blot baseddetection system for the presence <strong>of</strong> genes <strong>in</strong>volved <strong>in</strong> beta-lactam biosynthesis.Act<strong>in</strong>omycetol. 12(1): 29-36.Kuczek, K., Mordarski, M. <strong>and</strong> Goodfellow, M. (1994). Distribution <strong>of</strong> oxoacyl synthasehomology sequences with<strong>in</strong> Streptomyces DNA. FEMS Microbiol. Lett. 118:317-326.____________________________________________________________________________________150
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