Detection and Expression of Biosynthetic Genes in Actinobacteria ...

BERVANAKIS, G.__________________________________________________________________________________ Chapter 3: RESULTSPrimer act04f5’ GATGGTCTCCACCGGCTGC 3’451 540a.msf{AB024980} AGCACGCTCTCCTCCGTCGTGGC...CGAGCAGTTCGGCGCCCGC GGCCCGGTGCAGACGGTCTCCACCGGCTGCACCTCCGGCCTCGACa.msf{AB024981} AGCACCGTGGCCTCCGCTGTGGC...GGAACGTTTCGGCGCACGG GGTCCGGTGCAGACCGTCTCCACCGGCTGCACCTCCGGCCTGGACa.msf{AB024975} TCGTCGATGGCGGCGGAGATCGCCTGGCTG...GCCGAGGCGGAG GGCCCGGCCGGGGTGGTCTCGGCGGGCTGCACCTCGGGCATCGACa.msf{Z25538} TCGTCGATGGCGGCCGAGATCGCCTGGCTG...GCGGAGGCGGAG GGCCCGGCCGGGGTGGTCTCGGCCGGCTGCACCTCCGGCATCGACa.msf{AB024977} TCCTCCATGGTCAAGGAGATCGCCTGGCTG...GCCGGGGCCGAG GGCCCGGCGGGGGTGGTCTCTGCGGGCTGCACCTCGGGGATCGACa.msf{AB024976} TCCTCGATGGCCACCGAACTGGCCTGGCTG...GTCGAGGCGGAG GGTCCGGTGGGCGTGGTGTCGACCGGATGCACCTCGGGGATCGACa.msf{AB024970} AGTTCGATGGCGGCCGAAGTGGCGTGGGAG...GCCGGCGCCGAG GGCCCCGCGGCGCTCGTCTCGGCGGGCTGCACCTCCGGTCTCGACa.msf{AB024971} AGTTCGATGGCGGCCGAAGTGGCGTGGGAG...GCCGGCGCCGAG GGCCCCGCGGCGCTCGTCTCGGCGGGCTGCACCTCCGGTCTCGACa.msf{AB024972} AGCTCGATGGCGGCGGAAGTCGCCTGGGAG...GCCGGGGCGGAG GGCCCCACCGCCCTCATCTCGGCGGGCTGCACCTCCGGCCTGGACa.msf{AB024973} AGCTCGATGGCGGCGGAGGTCGCCTGGGAG...GCCGGCGCCGAG GGCCCGGCCGCGCTCGTCTCGGCGGGCTGCACCTCCGGCCTCGACa.msf{AB024974} AGCTCCATGGCGGCCGAGCTCGCCTGGGAG...GCCGGCGCCGAA GGCCCCTGCACCGTCGTCTCGGCCGGCTGCACGTCCGGCCTCGACa.msf{AB021222} AGCTCGGTCGCCGCCGAGGTCGCC...CGC...GACAGCGCGCTG GGCTCGGTCGCCGTCGTGTCCACGGGCTGCACCTCGGGACTCGACa.msf{X89899} AGCTCCATGGCCGCCGAGGTCGCCTGGAGC...ATCGGTGCGCAG GGCCCCGTGGCGCTGATCTCCACGGGCTGCACCTCCGGTCTCGACa.msf{AF098965} GGGGTGATGCCGGCCGAGGTGGCCTGGAGC...GTGGGCGCCGAG GGCCCGGTGACGATGGTCTCCGACGGCTGCACCTCCGGCCTGGACa.msf{X16300} GGCGTCATGCCCGCCGAGGTCGCCTGGGCG...GCCGGCGCGGAG GGCCCGGTCACCATGGTCTCCGACGGCTGCACCTCGGGCCTGGACa.msf{X63449} AGCGTCATGCCGGCCGAGGTCGCCTGGGCG...GTCGGCGCCGAG GGCCCGGTCACGATGGTCTCCACCGGCTGCACCTCGGGCCTGGACa.msf{L26338} AGCTCGCTCGCCCGCGAGGTGGCCGGGGTG...ATCGGCGCGGAG GGGCCCGCGGCAGTCGTCTCCACCGGCTGCACCTCCGGCATCGACa.msf{AB024978} AGCTCCTTCGCCGCCGAGGTCGCCTGGGCG...GTGGGCGCCGAG GGCCCCAGCACCGTGGTCTCTACCGGCTGCACCTCGGGCATCGACa.msf{AF126429} AGCTCCTTCTCCGCCGAGGTCGCCTGGGCC...GTCGGCGCCGAG GGCCCCAACACCGTGGTCTCCACCGGCTGCACCTCCGGCCTCGACa.msf{Z11511} AGCTCCTTCGCCGCGGAGGTGGCCTGGGCG...GTCGGCGCCGAG GGACCCTCCACGGTCGTCTCCACCGGCTGCACCTCCGGCATCGACa.msf{L24518} AGTTCGTTCGCCGCGGAGGTCGCCTGGTCG...GTCGGCGCCGAG GGCCCGGCGACGGTGGTCTCCACCGGGTGCACGTCAGGGCTGGACa.msf{X77865} AGCTCCTTCGCGACCGAAGTGGCCTGGGCC...GTCGGCGCGGAA GGACCGGCGACGGTGGTGTCCACCGGCTGCACCGCCGGGATCGACa.msf{AB024979} AGTTCCCTCGCGGCCGAGGTCGCTTGGGA...CGTCGGCGCGGAG GGCCCCGTCGCGACGGTGTCCACCGGCTGTACGTCAGGCCTGGACa.msf{AJ224512} AGCTCCATCGCCGCCGAGGTGGCACACGACCGCATCGGCGCGGAG GGCCCCGTCAGCCTCGTGTCGACCGGGTGCACCTCGGGCCTGGACa.msf{AB019690} AGTTCGCTCGCCGCCGAGGTCGCCTGGGTGTG...CGGTGCGGAG GGTCCGGTCGCGCTGATCTCCACCGGCTGCACGTCCGGGCTGGACa.msf{X15312} ACCTCCATCTGCCGGGAGGTCGCCTGGGAG...GCGGGGGCCGAG GGACCGGTGACGGTGGTGTCGACCGGGTGCACCTCGGGCCTCGACConsensus ----C--T--C--C-GA--T-GC--GG--------CGG-GC-GAG GG-CC--------T-GT-TC--C-GGCTGCACCTC-GG--T-GACFigure 21. Comparison of the nucleic acid sequences of KSα genes. The 26 sequences were compared by the PILEUP method. Respective accession numbers areshown in brackets after species name. S.coelicolor (X63449)., S.nogalater (AB024981)., S.steffisburgenesis (AB024979)., S.albus (AB024978)., S.platensis (AB024977).,S.aureofaciens (AB024976)., S.albofaciens (AB024975)., S.purpurascens (AB024974)., S.tauricus (AB024973)., S.capoamus (AB024972)., S.galilaeus (AB024971).,S.bobili (AB024970)., S.griseus (X77865)., S.glaucescens (X15312)., S.violaceoruber (X16300)., S.rimosus (Z25538)., S.argillaceus (X89899)., A.verrucosospora(AB019690)., S.venezuelae (AB024980)., S.venezuelae (AF126429)., S.nogalater (AJ224512)., S.rochei (AB021222)., S.roseofulvus (L26338)., S.cinnamonensis (Z11511).,S.arenae (AF098965)., K.aridum (L24518).____________________________________________________________________________________________________________________75