Detection and Expression of Biosynthetic Genes in Actinobacteria ...

BERVANAKIS, G.Chapter 3: RESULTSTable 48. Summary of PCR screening on environmental isolates*.Isolate # Type I PKS Type II PKS Deoxysugar Beta-LactamA0096A0347A0350A0371A1113A1488A1664A1990A2010A2056A2226A2360A2376A2381A2702A2834A3014A3023A3675A3771-++-+-----++-++---++----++--+++-+---+++-+++++---++----+----------------+--------+ PCR Positive amplification of the correct sized product- PCR Negative no amplification of the correct sized product* Shaded boxes indicate confirmation of correct PCR product was carried out bypartial sequencing._____________________________________________________________________96