_____________________________________________________________________________________Cruz, R., Arias, M.E. <strong>and</strong> Soliveri, J. (1999). Nutritional requirements for the production<strong>of</strong> pyrazoloisoqu<strong>in</strong>ol<strong>in</strong>one antibiotics by Streptomyces griseocarneus NCIMB 40447.Appl. Microbiol. Biotechnol. 53(1):115-119.Cruz, R., Arias, M.E. <strong>and</strong> Soliveri, J. (2000). APHE-3, a spore-associated antibiotic <strong>of</strong>Streptomyces griseocarneus NCIMB 40447. Appl. Microbiol. Biotechnol. 53:480-483.Cullum, J., Flett, F. <strong>and</strong> Piendl, W. “Genetic <strong>in</strong>stability, deletions, <strong>and</strong> DNAamplification <strong>in</strong> Streptomyces species.” Genetics <strong>and</strong> molecular biology <strong>of</strong> <strong>in</strong>dustrialmicroorganisms. Ed. Hersberger, C.L., Queener, S.W. <strong>and</strong> Hegeman, G. Wash<strong>in</strong>gton,D.C. USA: American society for microbiology, 1988. 127-131.Cundliffe, E. (1989). How antibiotic-produc<strong>in</strong>g organisms avoid suicide. Mol. Microbiol.5(12):2861-2867.Dahod, S.K. “Raw materials selection <strong>and</strong> medium development for <strong>in</strong>dustrialfermentation processes.” Manual <strong>of</strong> <strong>in</strong>dustrial microbiology <strong>and</strong> biotechnology 2 nd Ed.Ed. Dema<strong>in</strong>, A.L <strong>and</strong> Davies, J.E. Wash<strong>in</strong>gton, D.C. USA: ASM Press, 1999. 213-220.Dairi, T., Hamano, Y., Igarachi, Y., Furumai, T. <strong>and</strong> Oki, T. (1997). Clon<strong>in</strong>g <strong>and</strong>nucleotide sequence <strong>of</strong> the putative polyketide synthase genes for pradimic<strong>in</strong>biosynthesis from Act<strong>in</strong>omadura hibisca. Biosci. Biotechnol. Biochem. 61(9):1445-1453.Dairi, T., Hamano, Y., Furumai, T. <strong>and</strong> Oki, T. (1999). Development <strong>of</strong> a self-clon<strong>in</strong>gsystem for Act<strong>in</strong>omadura verrucosospora <strong>and</strong> identification <strong>of</strong> polyketide synthase genesessential for production <strong>of</strong> the angucyclic antibiotic pradimic<strong>in</strong>. Appl. Environ.Microbiol. 65(6):2703-2709.Dalbøge, H. <strong>and</strong> Lange, L. (1998). Us<strong>in</strong>g molecular techniques to identify new microbialbiocatalysts. Trends Biotechnol. 16:265-277.Damiani, G., B<strong>and</strong>i, C., Siron, M., Magrassi, L <strong>and</strong> Fani, R. (1997). CircumVent thermalcycle sequenc<strong>in</strong>g <strong>of</strong> Eubacterial 16S ribosomal DNAs. The NEB Transcript. 8(2):15.Dary, A., Bourget, N., Girard, N., Simonet, J.M. <strong>and</strong> Decaris, B. (1992). Amplification <strong>of</strong>a particular DNA sequence <strong>in</strong> Streptomyces amb<strong>of</strong>aciens RP181110 reversibly prevencespiramyc<strong>in</strong> production. Res. Microbiol. 143(1):99-112.DeCarvalho, A. <strong>and</strong> Mol<strong>in</strong>ari, R. (1983). Identification <strong>of</strong> low-molecular- weight nucleicacid-related substances secreted by Streptomyces aure<strong>of</strong>aciens. Appl. Environ. Microbiol.46(3):762-764.Decker, H., Gaisser, S., Pelzer, S., Schneider, P., Westrich, L., Wohlleben, W. <strong>and</strong>Bechthold, A. (1996). A general approach for clon<strong>in</strong>g <strong>and</strong> characteriz<strong>in</strong>g dNDP-glucosedehydratase genes from act<strong>in</strong>omycetes. FEMS Microbiol. Lett. 141:195-201.____________________________________________________________________________________141
_____________________________________________________________________________________Dekleva, M. L., Titus, A. J. & Strohl, W. R. (1985). Nutrient effects on anthracycl<strong>in</strong>eproduction by Streptomyces peucetius <strong>in</strong> a def<strong>in</strong>ed medium. Can. J. Microbiol. 31, 287-294.Dekleva, M.L <strong>and</strong> Strohl, W.R. (1987). Glucose-stimulated acidogenesis byStrepromyces peucetius. Can. J. Microbiol. 33:1129-1132.Dema<strong>in</strong>, A.L. “Carbon source regulation <strong>of</strong> idiolite biosynthesis <strong>in</strong> act<strong>in</strong>omycetes.”Regulation <strong>of</strong> secondary metabolism <strong>in</strong> act<strong>in</strong>omycetes. Ed. Shapiro, S. Boca Raton,Florida: CRC Press, Inc, 1989. 127-133.Dema<strong>in</strong>, A.L. “Why do microorganisms produce antimicrobials?” Fifty years <strong>of</strong>antimicrobials: past perspectives <strong>and</strong> future trends: fifty-third symposium <strong>of</strong> the societyfor general microbiology. Ed. Hunter, P.A., Darby, G.K <strong>and</strong> Russell, N.J. New York,USA. 1995a. 205-228Dema<strong>in</strong>, A.L. “Microbial secondary metabolism: The new frontier.” Secondarymetabolism <strong>of</strong> microorganisms- contributions on research. Ed. Kuhn, W <strong>and</strong> Fiedler, H-P. Tüb<strong>in</strong>gen, Germany: Attempto Verlag, 1995b. 10-35.Dema<strong>in</strong>, A.L. <strong>and</strong> El<strong>and</strong>er, R.P. (1999). The β-lactam antibiotics: past, present, <strong>and</strong>future. Antonie van Leeuwen. 75:5-19.Dema<strong>in</strong>, A.L. <strong>and</strong> Fang, A. (1995). Emerg<strong>in</strong>g concepts <strong>of</strong> secondary metabolism <strong>in</strong>act<strong>in</strong>omycetes. Act<strong>in</strong>omycetol. 9(2):98-117.Dick, O., Onken, U. <strong>and</strong> Zeeck, A. (1994). Influence <strong>of</strong> oncreased dissolved oxygenconcrntration on productivity <strong>and</strong> selectivity <strong>in</strong> cultures <strong>of</strong> a colabomyc<strong>in</strong>-produc<strong>in</strong>gstra<strong>in</strong> <strong>of</strong> Streptomyces grise<strong>of</strong>lavus. Appl. Microbiol. Biotechnol. 41:373-377.Distler, J., Ebert, A., Mansouri, K., Pissowotzki, K., Stockmann, M <strong>and</strong> Piepersberg, W.(1987). Gene cluster for streptomyc<strong>in</strong> biosynthesis <strong>in</strong> Streptomyces griseus: nucleotidesequence <strong>of</strong> three genes <strong>and</strong> analysis <strong>of</strong> transcriptional activity. Nucleic Acids Res.15:8041-8056.Distler, J., Mansouri, K., Mayer, G., Stockmann, M. <strong>and</strong> Piepersberg, W. (1992).Streptomyc<strong>in</strong> biosynthesis <strong>and</strong> its regulation <strong>in</strong> Streptomycetes. Gene 115:105-111.Doi-Katayama, Y., Yoon, Y.J., Choi, C-Y., Yu, T-W., Floss, H.G <strong>and</strong> Hutch<strong>in</strong>son, C.R.(2000). Thioesterases <strong>and</strong> the premature term<strong>in</strong>ation <strong>of</strong> polyketide cha<strong>in</strong> elongation <strong>in</strong>rifamyc<strong>in</strong> B biosynthesis by Amycolatopsis mediterranei S699. J. Antibiot. 53(5):484-495.____________________________________________________________________________________142
- Page 1 and 2:
___________________________________
- Page 3 and 4:
___________________________________
- Page 5 and 6:
___________________________________
- Page 7 and 8:
___________________________________
- Page 9 and 10:
___________________________________
- Page 11 and 12:
___________________________________
- Page 13 and 14:
___________________________________
- Page 15 and 16:
___________________________________
- Page 17 and 18:
___________________________________
- Page 19 and 20:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 21 and 22:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 23 and 24:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 25 and 26:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 27 and 28:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 29 and 30:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 31 and 32:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 33 and 34:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 35 and 36:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 37 and 38:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 39 and 40:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 41 and 42:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 43 and 44:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 45 and 46:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 47 and 48:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 49 and 50:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 51 and 52:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 53 and 54:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 55 and 56:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 57 and 58:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 59 and 60:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 61 and 62:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 63 and 64:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 65 and 66:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 67 and 68:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 69 and 70:
BERVANAKIS, G.Chapter 1: INTRODUCTI
- Page 71 and 72:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 73 and 74:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 75 and 76:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 77 and 78:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 79 and 80:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 81 and 82:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 83 and 84:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 85 and 86:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 87 and 88:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 89 and 90:
BERVANAKIS, G.Chapter 2: MATERIALS
- Page 91 and 92:
BERVANAKIS, G.Chapter 3: RESULTSSec
- Page 93 and 94:
BERVANAKIS, G.Chapter 3: RESULTSseq
- Page 95 and 96:
BERVANAKIS, G.Chapter 3: RESULTSFig
- Page 97 and 98:
BERVANAKIS, G._____________________
- Page 99 and 100:
BERVANAKIS, G.Chapter 3: RESULTSwit
- Page 101 and 102:
BERVANAKIS, G.Chapter 3: RESULTSTab
- Page 103 and 104:
BERVANAKIS, G.Chapter 3: RESULTS3.1
- Page 105 and 106:
BERVANAKIS, G.Chapter 3: RESULTSof
- Page 107 and 108:
BERVANAKIS, G. Chapter 3: RESULTS18
- Page 109 and 110:
BERVANAKIS, G.Chapter 3: RESULTS3.1
- Page 111 and 112: BERVANAKIS, G.Chapter 3: RESULTSDes
- Page 113 and 114: BERVANAKIS, G.Chapter 3: RESULTS3.1
- Page 115 and 116: BERVANAKIS, G.Chapter 3: RESULTSTab
- Page 117 and 118: BERVANAKIS, G.Chapter 3: RESULTSTab
- Page 119 and 120: BERVANAKIS, G.Chapter 3: RESULTSOf
- Page 121 and 122: BERVANAKIS, G.Chapter 3: RESULTSTab
- Page 123 and 124: BERVANAKIS, G.Chapter 3: RESULTS3.2
- Page 125 and 126: BERVANAKIS, G.Chapter 3: RESULTSLan
- Page 127 and 128: BERVANAKIS, G.Chapter 3: RESULTSTab
- Page 129 and 130: BERVANAKIS, G.Chapter 3: RESULTSThe
- Page 131 and 132: BERVANAKIS, G.Chapter 3: RESULTS3.2
- Page 133 and 134: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 135 and 136: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 137 and 138: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 139 and 140: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 141 and 142: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 143 and 144: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 145 and 146: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 147 and 148: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 149 and 150: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 151 and 152: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 153 and 154: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 155 and 156: BERVANAKIS, G.Chapter 4: DISCUSSION
- Page 157 and 158: ___________________________________
- Page 159 and 160: ___________________________________
- Page 161: ___________________________________
- Page 165 and 166: ___________________________________
- Page 167 and 168: ___________________________________
- Page 169 and 170: ___________________________________
- Page 171 and 172: ___________________________________
- Page 173 and 174: ___________________________________
- Page 175 and 176: ___________________________________
- Page 177 and 178: ___________________________________
- Page 179 and 180: ___________________________________
- Page 181 and 182: ___________________________________
- Page 183 and 184: ___________________________________
- Page 185 and 186: ___________________________________
- Page 187 and 188: ___________________________________
- Page 189 and 190: ___________________________________
- Page 191 and 192: ___________________________________
- Page 193 and 194: ___________________________________
- Page 195 and 196: ___________________________________
- Page 197 and 198: ___________________________________
- Page 199 and 200: ___________________________________
- Page 201 and 202: ___________________________________
- Page 203 and 204: ___________________________________
- Page 205 and 206: ___________________________________
- Page 207 and 208: ___________________________________
- Page 209: BERVANAKIS, G. APPENDIX 1Appendix 1