th - 1988 - 51st ENC Conference
th - 1988 - 51st ENC Conference
th - 1988 - 51st ENC Conference
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164<br />
I MODIFICATIOH OF A BRUKER WH-300 SPECTP.OI~TEP. FOP.<br />
B.~OADBAI[D/nlGd POWER SOLID STATE IR~ EXPEP.II~E~ITS<br />
Virgil Simplaceanu (*) and Chien Ho<br />
Department of Biological Sciences, Carnegie Mellon University<br />
4400 Fif<strong>th</strong> Avenue, Pittsburgh, PA 15213<br />
A modification is described <strong>th</strong>at allows performing broadband/high power I~<br />
experiments on a commerci~l spectrometer originally designed for high resolution<br />
I~. in liquids.<br />
Additional P~F gating and four phase channels are provided, gated by a timing<br />
simulator under <strong>th</strong>e control of <strong>th</strong>e Aspect computer. One RF channel has amplitude<br />
control capability for spin locking experiments and can also be used for shaped pulse<br />
applications if driven by an arbitrary waveform generator. A !lenry ~adio tunable i kW<br />
amplifier and/or a 50W broadband ENI amplifier are used as transmitters. The standard<br />
preamplifier is replaced by a fast mecovery, non-saturating preamplifier.<br />
Full compatibility and easy switchover from standard configuration to high power<br />
and back is maintained. The standard Bruker ~. software can be used to control <strong>th</strong>e<br />
execution of (automated) experiments via microprograms.<br />
165 I<br />
/ , /<br />
IN VIVO PHOSPHOROUS-31 NMR STUDIES OF HUMAN BRAIN AT 1.5T<br />
~aplan D. # , Pa,chalingam K. + McEvoy J. +, Spiker D. +, Keshavan M.S.+, Wolf GE #, Pettegrew J. 4--<br />
"The Pittsburgh NMR Institute, 3260 Fif<strong>th</strong> Ave, Pittsburgh, PA 15213<br />
+Western Psychiatric Institute and Clinic, 3211 O'Hara St., Pittsburgh, PA 15213<br />
We have studied <strong>th</strong>e dorsal prefrontal cortex in eight human subjects by 31p NMR. All studies were<br />
conducted on a General Electric Signa Scanner coordinating bo<strong>th</strong> imaging and spectroscopic protocols.<br />
Spectroscopic localization wi<strong>th</strong>in <strong>th</strong>e prefrontal cortex was achieved by surface coil B 1 profiling, and confirmed<br />
by proton imaging. Peak areas were calculated for phospho-monoesters, or<strong>th</strong>o-inorganic phosphate,<br />
phospho-dicsters, phosphocreatine, and <strong>th</strong>e nucleotide phosphates via a computerized spectral deconvolution<br />
program yielding a simulated spectrum of Iorentzian lines wi<strong>th</strong> frequencies, linewid<strong>th</strong>s, and areas<br />
corresponding to <strong>th</strong>e experimental spectrum. The observed values from normal adult volunteers, given in<br />
mole percent, are as follows: PME = 15.9, Pi = 8.06, PDE = 38.65. PCr = 11.15, gamma-ATP (ionized<br />
ends) = 9.44, alpha-ATP (esterified ends) = 9.89, beIa-ATP = 6.86. These values yield <strong>th</strong>e f¢~llowing<br />
ratios: PME/PDE = 0.41, PCr/P i = 1.38, PCr/ATP = 1.62. These results compal" ~ very well to bo<strong>th</strong><br />
classical biochemical assay of freeze clnmped extracted mammalian brain, as well as "P NMR studies of<br />
freeze-clamped exhacled mammalian brain.<br />
These results suggest <strong>th</strong>at in vivo NMR spectroscopy of adult human brnin conld provide metabolic<br />
insights into neuropsychiatric diseases. Schizophrc,ia, in particular, would be particulnrly applicable since<br />
bo<strong>th</strong> structural and metabolic alterations of <strong>th</strong>e dorsal prefrontal cortex have been implicated.<br />
181
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