th  - 1988 - 51st ENC Conference

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8:30 a.m. 8:55 a.m. 9:20 a.m. 9:30 a.m. 9:40 a.m. 10:05 a.m. THURSDAY MORNING ORDERED BIOLOGICAL SYSTEMS L. Batchelder, Session Chair "Tethered" Biological Systems: Results from NMR Spectroscopy. *L. Jelinski, R. W. Behling, D. Live, T. Yamane. Multinuclear Experiments for the Determination of Oligo- saccharide Structure in Liquid Crystal Phases. *J. H. Prestegard, P. Ram, L. T. Mazzola. Dynamics of the Gramicidin A Transmembrane Channel by Solid State lSN NMR. L. K. Nicholson, M. T. Brenneman, P. V. Lograsso, *T. A. Cross. Natural Abundance 13C and 14N NMR of Bacterial Osmolytes in vivo. *B. A. Lewis, S. C. Cayley, S. Padmanabhan, M. T. Record. NMR Studies of Anti-Spin Label Monoclonal Antibodies. *G. S. Rule, H. M. McConnell. Break. 60
THU 8:30 "TETHERED" BIOLOGICAL SYSTEMS: RESULTS FROM NMR SPECTROSCOPY Lynn W. Jelinski," Ronald W. Behling, David Live, + and Tetsuo Yamane, AT&T Bell Laboratories, Murray Hill, NJ 07974. By "tethered" biological systems, we mean assemblies in which two or more molecules arc either covalently or transiently joined together for the purpose of biological action. Tethered systems are a recurring theme in biophysics; examples include enzyme - subswate complexes, DNA - protein interactions, receptor - ligand binding, and antigen - antibody recognition. NMR spectroscopy is exceptionally well-suited to address key questions regarding both site - site recognition and chain folding in tethere.d biological assemblies. We will briefly describe three examples of tethered assemblies. In the first, polymer - peptide hybrids were constructed of polystyrene onto which was grown oligoglycines with varying but monodisperse chain lengths. Using solid state deuterium NMR spectroscopy, we showed that peptidc - peptide association occurred when the chain lengths were sufficient to form one overlap of the polyglycine II triple helical repeat. In another example, high resolution proton NMR was used to study the binding of small ligands to the acetylcholine receptor. Once the strategy was in place for observation of binding by NMR, 2D-NOE experiments were performed to determine the conformation of acetylcholine in its receptor-bound state. The results show that the conformation of the receptor in the bound state is significantly different from that when free in solution, suggesting that structure-activity relationships based solely on X-ray or solution conformations must be approached with caution. We are presently attempting to answer the question of whether nucleation sites for protein folding are formed during protein synthesis. To answer this question, we are preparing "fake" ribosomes (polyacrylamide) onto which the initial peptide segments of the S-peptide of ribonuclease are attached. These "fake" ribosomes are designed to mimic the way in which a protein would be synthesized in the cell. These tethered structures will be compared to the NMR spectra of the S-peptide, whose solution state conformation is being determined. Taken together, our results suggest that a combination of modem NMR techniques and cleverly chosen systems will have a substantial impact on our understanding of the structure and function of complex hybrid biological systems. + Department of Chemistry, Emery University, Atlanta, GA 30322 61
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29 th �� - 1988 Rochester
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PROGRAM: This conference program ha
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The Executive Committee of the 29th
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A A 0 0 ,-..1 I Z Z 0 "c N "1" i 0
Page 9 and 10: Restaurants in Rochester Restaurant
Page 11 and 12: 8:30 a.m. 8:35- 10:05 10:05 - 10:25
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Page 15 and 16: f~ I UNTRUNCATIO~I OF DIPOLE-DIPOLE
Page 17 and 18: MON 9:25 HIGH RESOLUTION ELECTROPHO
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Page 21 and 22: ~'-O-i 0 N i0:55 MEASUREMENTS OF TW
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Page 27 and 28: HON Eve 8:00 The 13C Relaxation Beh
Page 29 and 30: THE WORLD AND WONDERS OF 3H NMR SPE
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Page 33 and 34: TWO DIMENSIONAL LINEAR PREDICTION N
Page 35 and 36: TUE 9"20 ] 2D ROTATING FRAME SPECTR
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Page 47 and 48: TUE Eve 8-50 } Cu NQR OF YBa2Cu30 x
Page 49 and 50: ~X [I'IED 8:30 I REMOVAL OF EXTRANE
Page 51 and 52: . r WED 9:25 ] A STATIC NMR IMAGE O
Page 53 and 54: WED 9" 45 IFLUID AND SOLID STATE NM
Page 55 and 56: i MULTIVOLUME SELECTIVE SPECTROSCOP
Page 57 and 58: [WED 11-10 I SELF SHIELDED GRADIENT
Page 59: [WED 11:45 ] ANGIOGRAPHY USING MAGN
Page 63 and 64: • / I DYNAMICS OF THE GRAMICIDIN
Page 65 and 66: I THU 9:40 I NMR STUDIES OF ANTI-SP
Page 67 and 68: THU 10"2S I NEW TECHNIQUES POR DYNA
Page 69 and 70: I THU 11 : 05 I DYNAMIC NUCLEAR POL
Page 71 and 72: THIS SECTION CONTAINS A LISTING OF
Page 73 and 74: 9 ELIMINATION OF PHASE ROLL, SOLVEN
Page 75 and 76: 25 NUCLEAR MAGNETIC RESONANCE STUDI
Page 77 and 78: ~k 41 A HYPO-RELAXATION AGENT; SIMU
Page 79 and 80: 57 STRUCTURAL STUDIES OF LIPIDS IN
Page 81 and 82: 73 THE AUTOMATED NMR LABORATORY; SP
Page 83 and 84: 89 13C NMR ASSIGNMENTS OF DNA OLIGO
Page 85 and 86: 105 EVALUATION OF DOUBLE TUNED CIRC
Page 87 and 88: 121 BROADBAND SPIN DECOUPLING IN TH
Page 89 and 90: 137 TWO-DIMENSIONAL 13C{15N}, 13C{1
Page 91 and 92: 153 NMR CHARACTERIZATION OF THE SOL
Page 93 and 94: 169 SPECTROSCOPY WITH EXACT APODIZA
Page 95 and 96: 185 PARSING THE EDITED 1H NMR SIGNA
Page 97 and 98: . z 201 THE CORRELATION OF 1H-19F C
Page 99 and 100: i m 3 I 31p SOLID STATE NMR STUDIES
Page 101 and 102: CALCULATION OF 2951MAS NMR CHEMICAL
Page 103 and 104: AN NMR STUDY OF MISCIBLE BLENDS IN
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Page 107 and 108: 19 I DIPOLARAND SPIN-ROTATION POLAR
Page 109 and 110: 2 3- I A SOLID-STATE 2H and 13C NMR
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27 1 COLLECTION OF PHOSPHORUS-31 NM
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31 DELAYED REFOCUSSING TWO-DIMENSIO
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35 DYNAMIC NUCLEAR POLARIZATION STU
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I 39 2D NMR STUDIES AT 600 MHZ OF A
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43 Coherent Averaging Theory Under
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46 CARBON-13 SPECTRAL ASSIGNMENTS O
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a b 48 SEMUT SPECTRAL EDITING, CALI
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'--- V 5 2 j INTERCONWERSION OF VAL
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[ ~ THE SOURCE OF AN ARTIFACT IN TH
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60 GLUCOSE METABOLISM IN PERFUSED H
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. °- F 64 I MICROSCOPIC IMAGING OF
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68 69 CONFORMATIONAL ANALYSIS via V
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72 I SCUBA, A MAY TOHARDS COMPLETE
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76 NMR CHARACTERIZATION OF THE GLYP
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8o I HIGH RESOLUTION MR IMAGING AT
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A PROBE WITH HIGHER DECOUPLING EFFI
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88 I SOLID STATE ll3cd I~CLEAR }~GE
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- - 92 l CHARACTERIZATION OF NORMAL
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96 AN EVALUATION OF NEW PROCESSING
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. . I00 I NOVEL RESONATOR DESIGNS,
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104 TAYLOR TRANSFORMATION OF 2D NMR
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108 PERFORMANCE COMPARISON OF DOUBL
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RECENT EXTENSIONS OF NOESYSIM, A PR
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116 ] NODIFICATIONS TO A JEOL GX270
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I 12 o I SPECIATION OF WATER IN GLA
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124 I A COMPLETELY INTEGRATED NETWO
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I-- 1 2 8 IVOLUME-SELECTIVE SIGNAL
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-- is 2 I NATURAL ABUNDANCE 13 C an
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. ° THREE-DIMENSIONAL STRUCTURE OF
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~-'-- 140 DIRECT OBSERVATION OF LON
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144 I I IH AND 13C REFOCUSED GRADIE
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~ (POSTER ABSTRACT) BARBARA LYONS/C
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1 52 I ~IR STUDY OF NAPHTHALENE TRA
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is6 I ROTATING FRAME COHERENCE TRAN
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16 0 I A 27AL MAS STUDY OF AMORPHOU
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164 I MODIFICATIOH OF A BRUKER WH-3
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IN VIVO 31p AND IH NMR SPECTROSCOPY
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. r 172 JMAGIC ANGLE SPINNING SEPAR
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2 . ° I- 176 I Michael A. Kennedy
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. o . o 180 l17o/Is NMR MICROSCOPY
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To be presented at the 29th Experim
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INHIBITION OF ALANINE RACEMASE BY T
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192 RESOLUTION ENHANCEMENT OF PHOSP
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196 1 TR FLUOROETHOXY DERIVATIVES:
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200 INTERPRETAT IION OF 13 C NHR MI
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204 RECENT PROGRESS IN HIGH RESOLUT
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Page No. ACEVEDO, H F 165 ACKERMAN,
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1:~ge No. GUO, D 200 GUO, W 196 GUO
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OPELLA, S J ORENDT, A M OTTING, G P
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ZLOTNIK-MAZORIo T ZUMBULYADIS, N Pa
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David B. Bailey USI Chemicals Co. 1
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Yvan Boulanger Univ de Montreal Ins
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Helga Cohen Univ of So Carolina Che
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F. David Doty Doty Scientific Inc.
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Alan Freyer Pennsylvania State Univ
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Gordon Hamer Xerox Research Center-
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Timothy Hyman Syracuse University 3
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Robert A Kinsey BF Goodrich 9921Bre
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Jay J. Listinsky U of Rochester-Dep
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Dale Mierke Univ of Calif, San Dieg
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Jeanne C Owens Chemistry Dept., '.1
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Gade S. Reddy DuPont Experimental S
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Charles Schramm Catalytica Assoc. I
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Richard F. Sprecher U.S. Dept of En
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Steve Unger U. C. Davis NMR Facilit
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Toni Wirthlin Varian Associates 611
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th  - 1988 - 51st ENC Conference

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