th - 1988 - 51st ENC Conference
th - 1988 - 51st ENC Conference
th - 1988 - 51st ENC Conference
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Wed 11:55<br />
QUANTIFICATION OF BLOOD FLOW AND TISSUE PERFUSION VIA DEUTERIUM<br />
I NMR-THE NOVEL USE OF D=O AS A FREELY DIFFUSIBLE TRACER:<br />
Joseph J.H. Ackerman i W , Seong-Ci Kim*, Coleen S. Ewy*, Nancy N.<br />
56cker*, Yuying C. Hwang*, and Robert A. Shalwitz2; Departments of Chemistry* and<br />
Pediatrics 2, Washington University, St. Louis, MO 631301 and 631102 .<br />
NMR has proven to be a valuable technique wi<strong>th</strong> which to monitor metabolic events<br />
nondestructively in intact biological systems. The past decade has witnessed dramatic<br />
advances in <strong>th</strong>e development of such spectroscopic analyses employing alp, 13C, and *H<br />
nuclides. Our laboratory has recently introduced a new approach, employing deuterium<br />
NMR in concert wi<strong>th</strong> D20 as a freely diffusible aqueous tracer, for <strong>th</strong>e measurement of<br />
blood flow and tissue perfusion I'2 This me<strong>th</strong>od borrows heavily from multicompart-<br />
ment kinetic modeling used wi<strong>th</strong> diffusible radiotracers such as H2*SO and 133Xe but,of<br />
course, does not require <strong>th</strong>e special handling procedures associated wi<strong>th</strong> radioactive<br />
labels. In addition, <strong>th</strong>e deuterium NMR blood flow determination can be carried out<br />
concomitant wi<strong>th</strong> NMR metabolic analysis, <strong>th</strong>us, correlating in one measurement impaired<br />
substrate delivery and its physiologic consequences. In brief, <strong>th</strong>e tissue or organ in<br />
which blood flow is to be determined is labeled wi<strong>th</strong> D20 via ei<strong>th</strong>er intravenous, intra<br />
arterial or intratissue bolus injection. Ongoing capillary blood flow, diffusion and<br />
proton-deuteron exchange serve to distribute HOD <strong>th</strong>roughout <strong>th</strong>e tissue's aqueous space<br />
Fur<strong>th</strong>er blood flow (unlabeled) <strong>th</strong>en washes out <strong>th</strong>e deuterium residue. The residue<br />
decay (washout) curve is accurately defined via external monitoring, i.e., 2H NMR.<br />
Single*, ~ and multicompartment modeling 3'4 and knowledge of <strong>th</strong>e blood:tissue<br />
partition coefficient (readily determined independently of <strong>th</strong>e NMR residue decay curve<br />
allows derivation of blood flow and perfusion in units of ml-blood/(lO0 g-tissue,min).<br />
The extension of <strong>th</strong>is me<strong>th</strong>od to NMR flow-imaging appears feasible s . [References: (i)<br />
J.J.H. Ackerman et al., Proc. Natl. Acad. Sci. USA, 84, 4099 (1987); (2) J.J.H.<br />
Ackerman et al., N.Y. Acad. Sci., 508, 89 (1987); (3) S.-G. Kim et al., Cancer<br />
Research, accepted (1987); (4) S.-G. Kim, et al., Magn. Reson..Med., submitted<br />
(1987); (5) C.S. Ewy eC al., Magn. Reson. Med., submitted (1987).]<br />
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