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Microbiology, 2021

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3.3 • Unique Characteristics of Prokaryotic Cells 89<br />

Figure 3.15<br />

hypotonic environments.<br />

In cells that lack a cell wall, changes in osmotic pressure can lead to crenation in hypertonic environments or cell lysis in<br />

Figure 3.16<br />

In prokaryotic cells, the cell wall provides some protection against changes in osmotic pressure, allowing it to maintain its<br />

shape longer. The cell membrane is typically attached to the cell wall in an isotonic medium (left). In a hypertonic medium, the cell<br />

membrane detaches from the cell wall and contracts (plasmolysis) as water leaves the cell. In a hypotonic medium (right), the cell wall<br />

prevents the cell membrane from expanding to the point of bursting, although lysis will eventually occur if too much water is absorbed.<br />

CHECK YOUR UNDERSTANDING<br />

• Explain the difference between cell morphology and arrangement.<br />

• What advantages do cell walls provide prokaryotic cells?<br />

The Nucleoid<br />

All cellular life has a DNA genome organized into one or more chromosomes. Prokaryotic chromosomes are<br />

typically circular, haploid (unpaired), and not bound by a complex nuclear membrane. Prokaryotic DNA and<br />

DNA-associated proteins are concentrated within the nucleoid region of the cell (Figure 3.17). In general,<br />

prokaryotic DNA interacts with nucleoid-associated proteins (NAPs) that assist in the organization and

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