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Microbiology, 2021

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20.4 • EIAs and ELISAs 835<br />

Figure 20.24 (a) In a sandwich ELISA, a primary antibody is used to first capture an antigen with the primary antibody. A secondary<br />

antibody conjugated to an enzyme that also recognizes epitopes on the antigen is added. After the addition of the chromogen, a<br />

spectrophotometer measures the absorbance of end product, which is directly proportional to the amount of captured antigen. (b) An<br />

ELISA plate shows dilutions of antibodies (left) and antigens (bottom). Higher concentrations result in a darker final color. (credit b:<br />

modification of work by U.S. Fish and Wildlife Service Pacific Region)

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