Microbiology, 2021

13.2 • Using Physical Methods to Control Microorganisms 521
Case in Point
A Streak of Bad Potluck
One Monday in spring 2015, an Ohio woman began to experience blurred, double vision; difficulty
swallowing; and drooping eyelids. She was rushed to the emergency department of her local hospital.
During the examination, she began to experience abdominal cramping, nausea, paralysis, dry mouth,
weakness of facial muscles, and difficulty speaking and breathing. Based on these symptoms, the hospital’s
incident command center was activated, and Ohio public health officials were notified of a possible case of
botulism. Meanwhile, other patients with similar symptoms began showing up at other local hospitals.
Because of the suspicion of botulism, antitoxin was shipped overnight from the CDC to these medical
facilities, to be administered to the affected patients. The first patient died of respiratory failure as a result
of paralysis, and about half of the remaining victims required additional hospitalization following antitoxin
administration, with at least two requiring ventilators for breathing.
Public health officials investigated each of the cases and determined that all of the patients had attended
the same church potluck the day before. Moreover, they traced the source of the outbreak to a potato salad
made with home-canned potatoes. More than likely, the potatoes were canned using boiling water, a
method that allows endospores of Clostridium botulinum to survive. C. botulinum produces botulinum
toxin, a neurotoxin that is often deadly once ingested. According to the CDC, the Ohio case was the largest
botulism outbreak in the United States in nearly 40 years. 6
Killing C. botulinum endospores requires a minimum temperature of 116 °C (240 °F), well above the
boiling point of water. This temperature can only be reached in a pressure canner, which is recommended
for home canning of low-acid foods such as meat, fish, poultry, and vegetables (Figure 13.11). Additionally,
the CDC recommends boiling home-canned foods for about 10 minutes before consumption. Since the
botulinum toxin is heat labile (meaning that it is denatured by heat), 10 minutes of boiling will render
nonfunctional any botulinum toxin that the food may contain.
Figure 13.11 (a) Clostridium botulinum is the causative agent of botulism. (b) A pressure canner is recommended for home canning
because endospores of C. botulinum can survive temperatures above the boiling point of water. (credit a: modification of work by
Centers for Disease Control and Prevention; credit b: modification of work by National Center for Home Food Preservation)
6 CL McCarty et al. “Large Outbreak of Botulism Associated with a Church Potluck Meal-Ohio, 2015.” Morbidity and Mortality
Weekly Report 64, no. 29 (2015):802–803.