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Lead Toxicity in Mute Swans

LEAD TOXICITY IN MUTE SWANS Cygnus olor (Gmelin). By JOHN O'HALLORAN A thesis submitted to the National University of Ireland in candidature for the degree of Doctor of Philosophy September 1987

LEAD TOXICITY IN MUTE SWANS
Cygnus olor (Gmelin).
By
JOHN O'HALLORAN
A thesis submitted to the National University of Ireland
in candidature for the degree of Doctor of Philosophy
September 1987

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the maximum tolerable limit for lead was calculated as follows:<br />

2.20umoles/L X 207 1<br />

142<br />

- 3.00 ug Pb/ g Rb.<br />

1 =atomic weight of lead.<br />

thus, values of whole blood lead > 3.00ug Pb/g Rb is considered<br />

elevated. Similarily values of greater than 1.07 umoles/L (60.00ug/<br />

lOOmls) protoporphyr<strong>in</strong> have been adopted as <strong>in</strong>dicative of excess lead<br />

exposure after Bush~ al., (1982).<br />

On the same . basis as lead, the<br />

limit can be calculated as follows:<br />

2<br />

l.07umoles X 5612<br />

142<br />

molecular weight of PPIX.<br />

- 4.00 ug PPIX/ gRb<br />

thus samples with <strong>in</strong> excess of 4.00 ugPPIX/ gRb are considered to have<br />

been exposed to elevated lead.<br />

Age and physiological state were taken<br />

<strong>in</strong>to consideration for values of protoporphyr<strong>in</strong> IX and haemoglob<strong>in</strong> after<br />

O'Ralloran ~al.,<br />

(b, <strong>in</strong> press).<br />

Statistics<br />

All statistical calculations were carried out us<strong>in</strong>g a M<strong>in</strong>itab<br />

statistical package (Pennsylvania University, U.S.A.) on a Vax VMS.<br />

A<br />

B.M.D.P. PLR stepwise logistic regression analysis was used to exam<strong>in</strong>e<br />

the observed pattern of the proportion of birds of each class and age<br />

with elevated lead at Cork Lough.<br />

Medians and 25% percentiles were<br />

calculated to describe the distribution of blood lead levels over the<br />

two year sampl<strong>in</strong>g period.<br />

- 22 -<br />

I<br />

r

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