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6TargetsofSI 89<br />

is crosstalk between the SI signalling cascades and that PCD probably functions<br />

as a decision-making point that results in incompatible pollen being<br />

committed to death.<br />

Thus, we are beginning to dissect a complex network of signals and<br />

targetsinvolvedinsignallingcascadestriggeredbySI.Thechallengefor<br />

the future is not only to identify additional components, but also to elucidate<br />

how these components interact and what crosstalk is utilized during<br />

SI. In particular, the specific molecular interactions between [Ca 2+ ]i,actin<br />

alterationsandPCDsignallingcascadesarecurrentlybeinginvestigated.<br />

Furthermore, hints that p56 MAP kinase activation may also cross-talk to<br />

these elements are being pursued, as there is evidence for MAPK involvement<br />

in F-actin alterations and PCD in other systems (Zhang et al. 2000;<br />

Yang et al. 2001; Samaj et al. 2002). As the title of this book implies, it<br />

appears that plants have some aspects of a neuronal-like system of communication<br />

and we are just beginning to scratch the surface of understanding<br />

the intricacies of how cellular responses are achieved through networks of<br />

signalling intermediates.<br />

Acknowledgements. Research in the laboratory of V.E.F.-T. is supported<br />

by Biotechnology and Biological Sciences Research Council (BBSRC). Research<br />

in the laboratory of C.J.S. is supported by the US Department of<br />

Agriculture, National Research Initiative grant no. 2002–35304–12412, and<br />

by the US Department of Energy – Energy Biosciences Division grant DE-<br />

FG02-04ER15526.<br />

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Adrain C, Martin SJ (2001) The mitochondrial apoptosome: a killer unleashed by the<br />

cytochrome seas. Trends Biochem Sci 26:390–397<br />

Allwood EG, Smertenko AP, Hussey PJ (2001) Phosphorylation of plant actindepolymerising<br />

factor by calmodulin-like domain protein kinase. FEBS Lett 499:97–100<br />

Cardenas L, Vidali L, Dominguez J, Perez H, Sanchez F, Hepler PK, Quinto C (1998) Rearrangement<br />

of actin microfilaments in plant root hairs responding to Rhizobium etli<br />

nodulation signals. Plant Physiol 116:871–877<br />

Cheung AY, Wang H, Wu HM (1995) A floral transmitting tissue-specific glycoprotein<br />

attracts pollen tubes and stimulates their growth. Cell 82:383–393<br />

Clarke A, Desikan R, Hurst RD, Hancock JT, Neill SJ (2000) NO way back: nitric oxide and<br />

programmed cell death in Arabidopsis thaliana suspension cultures. Plant J 24:667–677<br />

Danon A, Rotari VI, Gordon A, Mailhac N, Gallois P (2004) Ultraviolet-C overexposure<br />

inducesprogrammedcelldeathinArabidopsis, which is mediated by caspase-like activities<br />

and which can be suppressed by caspase inhibitors, p35 and defender against<br />

apoptotic death. J Biol Chem 279:779–787<br />

de Graaf BHJ, Knuiman BA, Derksen J, Mariani C (2003) Characterization and localization<br />

of the transmitting tissue-specific PELPIII proteins of Nicotiana tabacum. JExpBot<br />

54:55–63

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