References

200 M. Gilliham et al.
13.4.5
NSCC Characterisation
The inadequacy of NSCC characterisation in planta and the uncertainty
regarding the definitive identification of ions involved in (or the signalling
components downstream of) the ligand gated Em depolarisation in
planta make it difficult to match the characteristics of putative heteromerdependentAtGLRcurrentstonativecurrents.Theuseofionchannelblockers
and ion substitution in microelectrode impalements are producing
some insights (Sivaguru et al. 2003; Qi et al. 2005) and greater resolution
could be provided by further patch clamp experiments (Demidchik et al.
2004) where greater control of solutions could also reveal whether cytosolic
components are involved. Selection of fluorescent protoplasts expressing
marker:AtGLR promoter fusions indicating expression of specific AtGLRs
within protoplasts may allow the tentative identification of currents with
gene products (Kiegle et al. 2000). Alternatively, mRNA could be extracted
from protoplasts after electrophysiological investigation and AtGLR transcript
abundance correlated with membrane conductance (Gehwolf et al.
2002).
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